Abstract
An ammonia process was applied at several ammonia loadings, moisture contents, temperatures, and dwell times. A cellulase loading of 5 FPU /g dry matter and a 24 h incubation time were used to produce the sugars, which were measured as reducing sugars and by HPLC. Optimal processing conditions caused a 76% of theoretical yield (2.9-fold above untreated). Cellulose and hemicellulose conversions were 68 and 85% (vs 38 and 34% in untreated, respectively). The short hydrolysis time and relatively low enzyme loading suggests great potential to produce sugars from alfalfa.
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Ferrer, A., Byers, F.M., Sulbarán-de-Ferrer, B. et al. Optimizing ammonia processing conditions to enhance susceptibility of legumes to fiber hydrolysis. Appl Biochem Biotechnol 98, 123–134 (2002). https://doi.org/10.1385/ABAB:98-100:1-9:123
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DOI: https://doi.org/10.1385/ABAB:98-100:1-9:123