Skip to main content
SpringerLink
Log in

Adsorptive immobilization of intestinal brush border membrane on triton X-100-substituted sepharose 4B

  • Published:
Applied Biochemistry and Biotechnology Aims and scope Submit manuscript

Abstract

Triton X-100-substituted Sepharose 4B (Sepharose-TX) was used for adsorptive immobilization of intestinal brush border membrane using lactose-phlorizin hydrolase as a representative membrane enzyme. Limited heating of membrane preparations was found to enhance binding. This enhancement is concluded to be owing to a greater availability of the hydrophobic sites, as also confirmed by the 1-anilino-8-naphthalene sulfonate fluorescence studies, for interaction with Triton X-100 moieties on the support. The immobilized preparations obtained by this procedure were found useful in hydrolysis of lactose, involving lactose-phlorizin hydrolase, in continuous operations. It is suggested that the approach may be of general utility for immobilization of biologic membranes by interaction of their extramembrane structures using supports with appropriate hydrophobic groups.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Sanderman, H. (1982), Eur. J. Biochem. 127, 123–128.

    Article  Google Scholar 

  2. Sanderman, H. (1978), Biochim. Biophys. Acta 515, 209–237.

    Google Scholar 

  3. Maneri, L. R. and Low, P. S. (1988), J. Biol. Chem. 263, 16,170–16,178.

    CAS  Google Scholar 

  4. Haltia, T. and Freire, E. (1995), Biochim. Biophys. Acta 1241, 295–322.

    PubMed  Google Scholar 

  5. Klibanov, A. M. (1979), Anal. Biochem. 93, 1–25.

    PubMed  CAS  Google Scholar 

  6. Monsan, P. and Combes, D. (1988), Methods Enzymol. 137, 585–598.

    Google Scholar 

  7. Cheetam, P. S. J. (1985), in Handbook of Enzyme Biotechnology, 2nd ed., Wiseman, A., ed., Ellis Harwood, Chichester, pp. 54–146.

    Google Scholar 

  8. Habibi-Rezaei, M. and Nemat-Gorgani, M. (1997), Appl. Biochem. Biotechnol. 67, 99–115.

    Google Scholar 

  9. Habibi-Rezaei, M. and Nemat-Gorgani, M. (1998), Int. J. Biochem. Cell Biol. 30, 417–422.

    Article  PubMed  CAS  Google Scholar 

  10. Tagvaei, M., Khezre-Barati, S., Jalilvand, F. and Nemat-Gorgani, M. (2000), J. Biotechnol. 81, 107–112.

    Article  Google Scholar 

  11. Mantei, N., Villa, M., Enzler, T., Waker, H., Boll, W., James, P., Huziker, W., and Semenza, G. (1988), EMBO J. 7, 2705–2713.

    PubMed  CAS  Google Scholar 

  12. Ramaswamy, S. and Radhakrishnan, A. N. (1975), Biochim. Biophys. Acta 403, 446–455.

    PubMed  CAS  Google Scholar 

  13. Skovbjerg, H., Noren, O., Sjostrom, H., Danielsen, E. M., and Enevoldsen, B. S. (1982), Biochim. Biophys. Acta 707, 89–97.

    PubMed  CAS  Google Scholar 

  14. Nemat-Gorgani, M. and Karimian, K. (1984), Biotechnol. Bioeng. 26, 565–572.

    Article  CAS  Google Scholar 

  15. Azari, F. and Nemat-Gorgani, M. (1999), Biotechnol. Bioeng. 62, 193–199.

    Article  PubMed  CAS  Google Scholar 

  16. Azari, F., Hosseinkhani, S., and Nemat-Gorgani, M. (2001), Appl. Biochem. Biotechnol. 94, 265–277.

    Article  PubMed  CAS  Google Scholar 

  17. Hauser, H., Howell, K., Dawson, R. M. C., and Bowyer, D. E. (1980), Biochim. Biophys. Acta 602, 567–577.

    Article  PubMed  CAS  Google Scholar 

  18. Haase, W., Schafer, A., Murer, H., and Kinne, R. (1978), Biochem. J. 172, 57–62.

    PubMed  CAS  Google Scholar 

  19. Li, H. J. (1978), Method Cell. Biol. 18, 385–396.

    CAS  Google Scholar 

  20. Mejbeum-Katzenellenbogen, W. and Dobryszyka, W. M. (1959), Clin. Chim. Acta 4, 515–520.

    Article  Google Scholar 

  21. Ulbrich, V., Makes, J., and Jurecek, M. (1964), Coll. Czech. Chem. Commun. 29, 1466–1475.

    CAS  Google Scholar 

  22. Rivera-Sagaredo, A., Canada, F. J., Nieto, O., Jimenez-Barbero, J., and Martin-Lomas, M. (1992), Eur. J. Biochem. 209, 415–422.

    Article  Google Scholar 

  23. Dahlquist, A. (1964), Anal. Biochem. 248, 6777–6780.

    Google Scholar 

  24. Morimoto, T., Arpin, M., and Gaetani, S. (1983), Methods Enzymol. 96, 121–157.

    PubMed  CAS  Google Scholar 

  25. Kessler, M., Acuto, O., Storelli, C., Murer, H., Muller, M., and Semenza, G. (1978), Biochim. Biophys. Acta 506, 136–154.

    Article  PubMed  CAS  Google Scholar 

  26. Dolgikh, D. A., Gilmanshin, R. I., Brazhnikov, E. V., Bychkova, V. E., Semisotnov, G. V., Venyaminov, S. Y., and Ptitsyn, O. B. (1981), FEBS Lett. 136, 311–315.

    Article  PubMed  CAS  Google Scholar 

  27. Ptitsyn, O. B. (1995), Adv. Protein Chem. 47, 83–229.

    Article  PubMed  CAS  Google Scholar 

  28. Poklar, N., Lah, J., Salobir, M., Mocek, P., and Vesnaver, G. (1997), Biochemistry 36, 14,345–14,352.

    Article  CAS  Google Scholar 

  29. Dolginova, E. A., Roth, E., Siman, I., and Weiner, L. M. (1992), Eur. J. Biochem. 200, 663–670.

    Google Scholar 

  30. Kreimer, D. I., Shnyrov, V. Z., and Villar, E. (1995), Protein Sci. 4, 2349–2357.

    Article  PubMed  CAS  Google Scholar 

  31. Fernandez, P., Canada, J., Jimenez-Barbero, J., and Martin-Lomas, M. (1995), Carbohydr. Res. 271, 31–42.

    Article  PubMed  CAS  Google Scholar 

  32. van der Goot, F. G., Gonzales-Manas, J. M., Lakey, J. H., and Pattus, F. (1991), Nature 354, 408–410.

    Article  PubMed  ADS  Google Scholar 

  33. Bychkova, V. E., Berni, R., Rossi, G., Kutyshenko, V. P., and Ptisyn, O. B. (1992), Biochemistry 31, 7566–7571.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Author notes

  1. Mehran Habibi-Rezaei

    Present address: Faculty of Science, University of Tehran, Tehran, Iran

Authors and Affiliations

  1. Institute of Biochemistry and Biophysics, University of Tehran, PO Box 13145-1384, Tehran, Iran

    Mehran Habibi-Rezaei & Mohsen Nemat-Gorgani

Authors
  1. Mehran Habibi-Rezaei
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Mohsen Nemat-Gorgani
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Mohsen Nemat-Gorgani.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Habibi-Rezaei, M., Nemat-Gorgani, M. Adsorptive immobilization of intestinal brush border membrane on triton X-100-substituted sepharose 4B. Appl Biochem Biotechnol 97, 79–90 (2002). https://doi.org/10.1385/ABAB:97:2:079

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1385/ABAB:97:2:079

Index Entries

Search

Navigation