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Production of native and recombinant lipases by Candida rugosa

A review

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Abstract

The yeast Candida rugosa produces multiple lipase isoenzymes sharing high sequence homology but with some differences in their catalytic properties. The regulation of C. rugosa lipase (CRL) synthesis and secretion in C. rugosa obeys a complex pattern. Fermentation processes for both wildtype and mutant C. rugosa strains are available for lipase production. Native CRL preparations have been extensively used for biotransformations. However, their inherent mixture of isoforms with variable profiles complicates interpretation and brings into question the reproducibility achieved between preparations. Although heterologous CRLs gene expression had been hampered owing to a nonuniversal codon usage, recent advances have made heterologous CRLs available. This will expand and improve the industrial utility of CRLs even further. The purpose of this review is to provide a summary of the recent advances on the production of native and recombinant lipases by C. rugosa.

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References

  1. Jaeger, K. E., Ransac, S., Dijkstra, B. W., Colson, C., van Heuvel, M., and Misset, O. (1994), FEMS Microbiol. Rev. 15(1), 29–63.

    Article  CAS  Google Scholar 

  2. Jaeger, K. E. and Reetz, M. T. (1998), Tibtech 16(9), 396–403.

    CAS  Google Scholar 

  3. Bailie, P., McNerlen, S., Robinson, E., and Murphy, W. (1994), Appl. Biocatal. 14, 11–13.

    Google Scholar 

  4. Bjorkling, F., Godtfredsen, S. E., and Kirk, O. (1991), Tibtech 9(10)P, 360–363.

    Google Scholar 

  5. Pandey, A., Benjamin, S., Soccol, C. R., Nigam, P., Krieger, N., and Soccol, V. T. (1999), Biotechnol. Appl. Biochem. 29(Pt. 2), 119–131.

    CAS  Google Scholar 

  6. Benjamin, S. and Pandey, A. (1998), Yeast 14, 1069–1087.

    Article  CAS  Google Scholar 

  7. Schmidt-Dannert, C. (1999), Biorg. Med. Chem. 7(10), 2123–2130.

    Article  CAS  Google Scholar 

  8. Rogalska, E., Douchert, I., and Verger, R. (1997), Biochem. Soc. Trans. 25(1), 161–164.

    CAS  Google Scholar 

  9. Ghosh, P. K., Saxena, R. K., Gupta, R., Yadav, R. P., and Davidson, S. (1996), Sci. Prog. 79(Pt. 2), 119–157.

    CAS  Google Scholar 

  10. Jaeger, K. E., Dijkstra, B. W., and Reetz, M. T. (1999), Annu. Rev. Microbiol. 53, 315–351.

    Article  CAS  Google Scholar 

  11. Sarda, L. and Desnuelle, P. (1958), Biochim. Biophys. Acta 30, 513–521.

    Article  CAS  Google Scholar 

  12. Ransac, S., Ivanova, M., Panaiotov, I., and Verger, R. (1999), Methods Mol. Biol. 109, 279–302.

    CAS  Google Scholar 

  13. Momsen, W. E. and Brockman, H. L. (1997), Methods Enzymol. 286, 292–305.

    CAS  Google Scholar 

  14. Labourdenne, S., Cagna, A., Delorme, B., Esposito, G., Verger, R., and Riviere, C. (1997), Methods Enzymol. 286, 306–326.

    CAS  Google Scholar 

  15. Verger, R. (1997), Tibtech 15(1), 32–38.

    CAS  Google Scholar 

  16. Kierkels, J. G. T., Vleugels, L. F. W., Gelade, E. T. F., Vermeulen, D. P., Kamphuis, J., Wandrey, C., and Vandentweel, W. J. J. (1994), Enzyme Microb. Technol. 16(6), 513–521.

    Article  CAS  Google Scholar 

  17. Helistö, P. and Korpela, T. (1998), Enzyme Microb. Technol. 23(1–2), 113–117.

    Article  Google Scholar 

  18. Rua, M. L., Díaz-Mauriño, T., Fernández, V. M., Otero, C., and Ballesteros, A. (1993), Biochim. Biophys. Acta 1156(2), 181–189.

    CAS  Google Scholar 

  19. Jensen, R. E. (1983), Lipids 18(9), 650–657.

    Article  CAS  Google Scholar 

  20. Jaeger, K. E., Liebeton, K., Zonta, A., Schimossek, K., and Reetz, M. T. (1996), Appl. Microbiol. Biotechnol. 46(2), 99–105.

    Article  CAS  Google Scholar 

  21. Thomson, C. A., Delaquis, P. J., and Mazza, G. (1999), Crit. Rev. Food Sci. Nutr. 39(2), 165–187.

    Article  CAS  Google Scholar 

  22. Erdman, H., Vorderwülbecke, T., Schmid, R., and Kieslich, K. (1990), in Lipases: Structure, Mechanism and Genetic Engineering, vol. 16, Alberghina, L., Schmid, R. D., and Verger, R., eds., GBF monographys, VCH, Weinheim, Germany, pp. 425–428.

    Google Scholar 

  23. Rapp, P. and Backhaus, S. (1992), Enzyme Microb. Technol. 14(11), 938–943.

    Article  CAS  Google Scholar 

  24. Arpigny, J. L. and Jaeger, K. E. (1999), Biochem. J. 343, 177–183.

    Article  CAS  Google Scholar 

  25. White, J. S. and White, D. C. (1997), Source Book of Enzymes, CRC Press, New York.

    Google Scholar 

  26. Kawaguchi, Y. and Honda, H. (1990), in Lipases: Structure, Mechanism and Genetic Engineering, vol. 16, Alberghina, L., Schmid, R. D., and Verger, R., eds., GBF monographs, VCH, Weinheim, Germany, pp. 221–230.

    Google Scholar 

  27. Lotti, M., Grandori, R., Fusetti, F., Longhi, S., Brocca, S., Tramontano, A., and Alberghina, L. (1993), Gene 124(1), 45–55.

    Article  CAS  Google Scholar 

  28. Lotti, M., Tramontano, A., Longhi, S., Fusetti, F., Brocca, S., Pizzi, E., and Alberghina, L. (1994), Protein Eng. 7(4), 531–535.

    Article  CAS  Google Scholar 

  29. Lotti, M. and Alberghina, L. (1996), in Engineering of/with Lipases, Malcata, F. X., ed., Kluwer Academic, Dordrecht, The Netherlands, pp. 115–124.

    Google Scholar 

  30. Diczfalusy, M. A., Hellman, U., and Alexon, S. E. H. (1997), Arch. Biochem. Biophys. 348, 1–8.

    Article  CAS  Google Scholar 

  31. Sánchez, A., Ferrer, P., Serrano, A., Pernas, M. A., Valero, F., Rúa, M. L., Casas, C., and Solà, C. (1999), Enzyme Microb. Technol. 25(3–5), 214–223.

    Article  Google Scholar 

  32. Kaiser, R., Erman, M., Duax, W. L., Ghosh, D., and Jörnvall, H. (1994), FEBS Lett. 337(2), 123–127.

    Article  CAS  Google Scholar 

  33. Lee, G. C., Tang, S. J., Sun, K. H., and Shaw, J. F. (1999), Appl. Environ. Microbiol. 65(9), 3888–3895.

    CAS  Google Scholar 

  34. Mileto, D., Brocca, S., Lotti, M., Takagi, M., Alquati, C., and Alberghina, L. (1998), Chem. Phys. Lipids 93, 47–55.

    Article  CAS  Google Scholar 

  35. López, C., Guerra, N. P., and Rua, M. L. (2000), Biotechnol. Lett. 22, 1291–1294.

    Article  Google Scholar 

  36. Iwai, M. and Tsujisaka, Y. (1994), Agric. Biol. Chem. 38, 1241–1247.

    Google Scholar 

  37. Patkar, S. A., Bjorkling, F., Zundel, M., Schulein, M., Svendsen, A., and Heldt-Hansen, H. P. (1993), Ind. J. Chem. 32(Pt. 1), 76–80.

    Google Scholar 

  38. Ohnishi, K., Yoshida, Y., and Sekiguchi, J. (1994), J. Ferment. Bioeng. 77(5), 490–495.

    Article  CAS  Google Scholar 

  39. Catoni, E., Schmidt-Dannert, C., Brocca, S., and Schmid, R. D. (1997), Biotechnol. Tech. 11(9), 689–695.

    Article  CAS  Google Scholar 

  40. Fu, Y., Ibrahim, A. S., Fonzi, W., Zhou, X., Ramos, C. F., and Ghannoum, M. A. (1997), Microbiology 143, 331–340.

    CAS  Google Scholar 

  41. Grochulski, P., Li, Y., Schrag, J., Bouthillier, F., Smith, P., Harrison, D., Rubin, B., and Cygler, M. (1993), J. Biol. Chem. 268(17), 12,843–12,847.

    CAS  Google Scholar 

  42. Ghosh, D., Wawrzak, Z., Pletnev, V. Z., Li, N., Kaiser, R., Pangborn, W., Jornvall, H., Erman, M., and Duax, W. L. (1995), Structure 3, 279–288.

    Article  CAS  Google Scholar 

  43. Cygler, M. and Schrag, J. D. (1999), Biochim. Biophys. Acta 1441, 205–214.

    CAS  Google Scholar 

  44. Grochulski, P., Li, Y., Schrag, J. D., and Cygler, M. (1994), Protein Sci. 3(1), 82–91.

    Article  CAS  Google Scholar 

  45. Brocca, S., Persson, M., Wehtje, E., Adlercreutz, P., Alberghina, L., and Lotti, M. (2000), Protein Sci. 9, 985–990.

    CAS  Google Scholar 

  46. Tang, S. J., Sun, K. H., Sun, G. H., Chang, T. Y., and Lee, G. C. (2000), Protein Express. Purif. 20, 308–313.

    Article  CAS  Google Scholar 

  47. Lakshmi, B. S., Kangueane, B., Abraham, B., and Pennatur, G. (1999), Lett. Appl. Microbiol. 29(1), 66–70.

    Article  CAS  Google Scholar 

  48. Gordillo, M. A., Obradors, N., Montesinos, J. L., Valero, F., Lafuente, J., and Solà, C. (1995), Appl. Microbiol. Biotechnol. 43(1), 38–41.

    Article  CAS  Google Scholar 

  49. Valero, F., Ayats, F., López-Santín, J., and Poch, M. (1988), Biotechnol. Lett. 10(10), 741–744.

    Article  CAS  Google Scholar 

  50. Tsujisaka, Y., Iwai, M., Fukumoto, J., and Okamoto, Y. (1973), Agric. Biol. Chem. 37(4), 837–842.

    CAS  Google Scholar 

  51. Valero, F., del Río, J. L., Poch, M., and Solà, C. (1991), J. Ferment. Bioeng. 72(5), 399–401.

    Article  CAS  Google Scholar 

  52. Wang, Y., Luopa, J., Rajalahti, T., and Linko, S. (1995). Biotechnol. Tech. 9(10), 741–746.

    Article  CAS  Google Scholar 

  53. Sokolovska, I., Albasi, C., Riba, J. P., and Bales, V. (1998), Bioprocess Eng. 19(3), 179–186.

    CAS  Google Scholar 

  54. Emery, A. N., Jan, D. C. H., and Al-Rubeai, M. (1995), Appl. Microbiol. Biotechnol. 43(6), 1028–1033.

    Article  CAS  Google Scholar 

  55. Ota, Y., Miyairi, S., and Yamada, K. (1968), Agric. Biol. Chem. 32(12), 1476–1478.

    CAS  Google Scholar 

  56. Obradors, N., Montesinos, J. L., Valero, F., Lafuente, J., and Solà, C. (1993), Biotechnol. Lett. 15(4), 357–360.

    Article  CAS  Google Scholar 

  57. Dalmau, E., Montesinos, J. L., Lotti, M., and Casas, C. (2000), Enzyme Microb. Technol. 26(9–10), 657–663.

    Article  CAS  Google Scholar 

  58. Nahas, E. (1988), J. Gen. Microbiol. 134(Pt. 1), 227–233.

    CAS  Google Scholar 

  59. Baillargeon, M. W., Bistline, R. G., and Sonnet, P. E. (1989), Appl. Microbiol. Biotechnol. 30(1), 92–96.

    Article  CAS  Google Scholar 

  60. Rapp, P. (1995), Enzyme Microb. Technol. 17(9), 832–838.

    Article  CAS  Google Scholar 

  61. Lotti, M., Monticelli, S., Montesinos, J. L., Brocca, S., Valero, F., and Lafuente, J. (1998), Chem. Phys. Lipids 93, 143–148.

    Article  CAS  Google Scholar 

  62. Chang, R. C., Chou, S. J., and Shaw, J. F. (1994), Biotechnol. Appl. Biochem. 19(Pt 1), 93–97.

    CAS  Google Scholar 

  63. Montesinos, J. L., Obradors, N., Gordillo, M. A., Valero, F., Lafuente, J., and Solà, C. (1996), Appl. Biochem. Biotechnol. 59(1), 25–37.

    CAS  Google Scholar 

  64. Gradova, N. B., Belov, A. P., and Guselnikova, T. V. (1990), Acta Biotechnol. 10(2), 169–177.

    Article  CAS  Google Scholar 

  65. Gordillo, M. A., Sanz, A., Sánchez, A., Valero, F., Montesinos, J. L., Lafuente, J., and Solà, C. (1998), Biotechnol. Bioeng. 60(2), 156–168.

    Article  CAS  Google Scholar 

  66. Benjamin, S. and Pandey, A. (1996), Bioresour. Technol. 55(2), 167–170.

    Article  CAS  Google Scholar 

  67. Christakopoulos, P., Tzia, C., Kekos, D., and Macris, B. J. (1992), Appl. Microbiol. Biotechnol. 38(2), 194–197.

    Article  CAS  Google Scholar 

  68. Salleh, A. B., Musani, R., Basri, M., Ampon, K., Yunus, W. M. Z., and Razac, C. N. A. (1993), Can. J. Microbiol. 39(10), 978–981.

    Article  CAS  Google Scholar 

  69. Pokorny, D., Friedrich, J., and Cimerman, A. (1994), Biotechnol. Lett. 16(4), 363–366.

    Article  CAS  Google Scholar 

  70. Papaparaskevas, D., Christakopoulos, P., Kekos, D., and Macris, B. J. (1992), Biotechnol. Lett. 14(5), 397–402.

    Article  CAS  Google Scholar 

  71. Shimada, Y., Sugihara, A., Nagao, T., and Tominaga, Y. (1992), J. Ferment. Bioeng. 74(2), 77–80.

    Article  CAS  Google Scholar 

  72. Linko, Y. Y. and Wu, X. Y. (1996), J. Chem. Technol. Biotechnol. 65(2), 163–170.

    Article  CAS  Google Scholar 

  73. Sidebottom, C. M., Charton, E., Dunn, P. P., Mycock, G., Davies, C., Sutton, J. L., Macrae, A. R., and Slabas, A. R. (1991), Eur. J. Biochem. 202(2), 485–491.

    Article  CAS  Google Scholar 

  74. Bertolini, M. C., Laramee, L., Thomas, D. Y., Cygler, M., Schrag, J. D., and Vernet, T. (1994), Eur. J. Biochem. 219(1–2), 119–125.

    Article  CAS  Google Scholar 

  75. Pignède, G., Wang, H., Fudalej, F., Gaillardin, C., Seman, M., and Nicaud, J. M. (2000), J. Bacteriol. 182(10), 2802–2810.

    Article  Google Scholar 

  76. Ratledge, C. and Evans, C. T. (1989), in The Yeast, vol. 3, Rose, A. H. and Harrison, J. H., eds., Academic, New York, pp. 372–385.

    Google Scholar 

  77. Dalmau, E. (1999), PhD thesis, Universitat Autònoma de Barcelona, Barcelona, Spain.

    Google Scholar 

  78. Eisai Co., Ltd. (1976), Japanese patent JP 76 57,889.

  79. Mitsui Toatsu Chemicals Co., Ltd. (1970), Japanese patent JP 70 08,632.

  80. Meito Sangyo Co. Ltd. (1964), Japanese patent JP 2987 (′64).

  81. Amano-Pharm. (1990), Japanese patent JP J02086787.

  82. Amano-Pharm. (1988), Japanese patent JP J63304992.

  83. Meito-Sangyo Co., Ltd. (1987), Japanese patent JP J62019090.

  84. Meito-Sangyo Co. Ltd. (1983), Japanese patent JP J58051889.

  85. Mulchandani, A. and Bassi, A. S. (1995), Crit. Rev. Biotechnol. 15(2), 105–124.

    CAS  Google Scholar 

  86. Olsson L. and Nielsen, J. (1997), Tibtech 15(12), 517–522.

    CAS  Google Scholar 

  87. Valero, F., Poch, M., Solà, C., Santos Lapa, R. A., and Costa Lima, J. L. F. (1991), Biotechnol. Tech. 5(4), 251–254.

    Article  CAS  Google Scholar 

  88. Cos, O., Montesinos, J. L., Lafuente, J., Solà, C., and Valero, F. (2000), Biotechnol. Lett. 22, 1783–1788.

    Article  CAS  Google Scholar 

  89. Sánchez, A., Gordillo, M. A., Montesinos, J. L., Valero, F., and Lafuente, J. (1999), J. Biosci. Bioeng. 87(4), 500–506.

    Article  Google Scholar 

  90. Chattaway, T., Demain, A. L., and Stephanopoulos, G. (1992), Biotechnol. Prog. 8(1), 81–84.

    Article  CAS  Google Scholar 

  91. Heinzle, E. and Reuss, M. (1987), Mass Spectrometry in Biotechnological Process Analysis and Control, Plenum, New York.

    Google Scholar 

  92. Von Schalien, R., Fagervick, F., Saxén, B., Ringbom, K., and Rydström, M. (1995), Biotechnol. Bioeng. 48(6), 631–638.

    Article  Google Scholar 

  93. Montesinos, J. L., Campmajó, C., Iza, J., Valero, F., Lafuente, J., and Solà, C. (1993), Biotechnol. Tech. 7(7), 429–434.

    Article  CAS  Google Scholar 

  94. Montesinos, J. L., Campmajó, C., Iza, J., Valero, F., Lafuente, J., and Solà, C. (1994), Process Contr. Qual. 5, 237–244.

    CAS  Google Scholar 

  95. Cheruy, A. (1997), J. Biotechnol. 52(3), 193–199.

    Article  CAS  Google Scholar 

  96. Stephanopoulos, G. and San, K. Y. (1984), Biotechnol. Bioeng. 26(10), 1176–1188.

    Article  CAS  Google Scholar 

  97. Albiol, J., Campmajó, C., Casas, C., and Poch, M. (1993), Biotechnol. Prog. 11(1), 88–92.

    Article  Google Scholar 

  98. Van der Heijden, R. T. J. M., Hellinga, C., Luyben, K. C. A. M., and Honderd, G. (1989), Tibtech 7(8), 205–209.

    Google Scholar 

  99. Cazzador, L. and Lubenova, V. (1995), Biotechnol. Bioeng. 47(6), 626–632.

    Article  CAS  Google Scholar 

  100. Farza, M., Hammouri, H., Jallut, C., and Liéto, J. (1999), AIChE 45, 93–106.

    Article  CAS  Google Scholar 

  101. Ljung, L. and Soderstrom, T. (1983), Theory and Practice of Recursive Identification, MIT Press, Cambridge, MA.

    Google Scholar 

  102. Chattaway, T. and Stephanopoulos G. (1989), Chem. Eng. Sci. 44(1), 41–48.

    Article  CAS  Google Scholar 

  103. Thibault, V., Van Breusegem, V., and Cheruy, A. (1990), Biotechnol. Bioeng. 36(10), 1041–1048.

    Article  CAS  Google Scholar 

  104. Montague, G. and Morris, A. J. (1994), Tibtech 12(8), 312–324.

    CAS  Google Scholar 

  105. Linko, S., Zhu, Y. H., and Linko, P. (1999), Tibtech 17(4), 155–162.

    CAS  Google Scholar 

  106. Valero, F., Lafuente, J., Poch, M., and Solà, C. (1990), Appl. Biochem. Biotechnol. 24–25, 591–602.

    Article  Google Scholar 

  107. Montesinos, J. L., Lafuente, J., Gordillo, M. A., Valero, F., Solà, C., Charbonnier, S., and Cheruy, A. (1995), Biotechnol. Bioeng. 48(6), 573–584.

    Article  CAS  Google Scholar 

  108. Aynsley, M., Hofland, A., Morris, A. J., Montague, G. A., and Di Massimo, C. (1993), Adv. Biochem. Eng. Biotechnol. 48, 1–27.

    CAS  Google Scholar 

  109. Simutis, R., Dors, M., and Lübbert, A. (1995), J. Biotechnol. 42(3), 285–290.

    Article  CAS  Google Scholar 

  110. Tartakovsky, B., Ulitzur, S., and Sheintuch, M. (1995), Biotechnol. Prog. 11(1), 80–87.

    Article  CAS  Google Scholar 

  111. Gomersall, R., Hitzmann, D., and Guthke, R. (1997), Bioprocess Eng. 17(2), 69–73.

    Article  CAS  Google Scholar 

  112. Sargantanis, I. G. and Karim, M. N. (1998), Biotechnol. Bioeng. 60(1), 1–9.

    Article  CAS  Google Scholar 

  113. Saucedo, V. M. and Karim, M. N. (1997), Biotechnol. Bioeng. 55(2), 317–327.

    Article  CAS  Google Scholar 

  114. Ignatova, M., Lubenova, V., and Georgieva, P. (2000), Bioprocess Eng. 22(1), 79–84.

    Article  CAS  Google Scholar 

  115. Del Río, J. L., Serra, P., Valero, F., Poch, M., and Solà, C. (1990), Biotechnol. Lett. 12(11), 835–838.

    Article  Google Scholar 

  116. Serra, P., del Río, J. L., Robusté, J., Poch, M., Solà, C., and Cheruy, A. (1992), Bioprocess Eng. 8(3–4), 145–150.

    Article  CAS  Google Scholar 

  117. Montesinos, J. L., Gordillo, M. A., Valero, F., Lafuente, J., Solà, C., and Valdman, B. (1997), J. Biotechnol. 52(3), 207–218.

    Article  CAS  Google Scholar 

  118. Gordillo, M. A., Montesinos, J. L., Casas, C., Valero, F., Lafuente, J., and Solà, C. (1998), Chem. Phys. Lipids 93, 131–142.

    Article  CAS  Google Scholar 

  119. Lee, S. Y. and Rhee, J. S. (1993), Enzyme Microb. Technol. 15(7), 617–623.

    Article  CAS  Google Scholar 

  120. Rao, P. V., Jayaraman, K., and Lakshmanan, C. M. (1993), Process Biochem. 28(6), 385–389.

    Article  CAS  Google Scholar 

  121. Rao, P. V., Jayaraman, K., and Lakshmanan, C. M. (1993), Process Biochem. 28(6), 391–395.

    Article  CAS  Google Scholar 

  122. Benjamin, S. and Pandey, A. (1997), Acta Biotechnol. 17(3), 241–251.

    Article  CAS  Google Scholar 

  123. Ferrer, P. and Solà, C. (1992), Appl. Microbiol. Biotechnol. 37(6), 737–741.

    Article  CAS  Google Scholar 

  124. López, S., Valero, F., and Solà, C. (1996), Appl. Biochem. Biotechnol. 59(1), 15–24.

    Google Scholar 

  125. Benjamin, S. and Pandey, A. (1997), Process Biochem. 32(5), 437–440.

    Article  CAS  Google Scholar 

  126. Dalmau, E., Sánchez, A., Montesinos, J. L., Valero, F., Lafuente, J., and Casas, C. (1998), J. Biotechnol. 59(3), 183–192.

    Article  CAS  Google Scholar 

  127. Sánchez, A., Ferrer, P., Serrano, A., et al. (1999), J. Biotechnol. 69(2–3), 169–182.

    Article  Google Scholar 

  128. Sánchez, A., De la Casa, R. M., Sinisterra, J. V., Valero, F., and Sánchez-Montero, J. M. (1999), Appl. Biochem. Biotechnol. 80(1), 65–75.

    Article  Google Scholar 

  129. Weber, H. K., Stecher, H., and Faber, K. (1995), in Preparative Biotransformations, Robert, S. M., ed., Wiley, New York, pp. 5–21.

    Google Scholar 

  130. Barton, M. J., Hamman, J. P., Fichter, K. C., and Calton, C. J. (1990), Enzyme Microb. Technol. 12(8), 577–583.

    Article  CAS  Google Scholar 

  131. Tsai, S. W. and Dordick, J. S. (1996), Biotechnol. Bioeng. 52(2), 296–300.

    Article  CAS  Google Scholar 

  132. Lundell, K., Raijola, T., and Kanerva, L. T. (1998), Enzyme Microb. Technol. 22(2), 86–93.

    Article  CAS  Google Scholar 

  133. Veeraragavan, K. and Gibbs, B. (1989), Biotechnol. Lett. 11(5), 345–348.

    Article  CAS  Google Scholar 

  134. Brahimi-Horn, M. C., Guglielmino, M. L., Elling, L., and Sparrow, L. G. (1990), Biochim. Biophys. Acta 1042(1), 51–54.

    CAS  Google Scholar 

  135. Rothschild, N., Hadar, Y., and Dosoretz, C. (1997), Appl. Environ. Microbiol. 63(3), 857–861.

    CAS  Google Scholar 

  136. Wu, J. Y., Hanhove, J. L. K., Huang, Y. S., and Chen, Y. T. (1996), Biochem. Mol. Biol. Int. 39(4), 755–764.

    CAS  Google Scholar 

  137. Diczfalusy, M. A. and Alexson, S. E. H. (1996), Arch. Biochem. Biophys. 334, 104–112.

    Article  CAS  Google Scholar 

  138. Cirigliano, M. C. and Carman, G. M. (1984), Appl. Environ. Microbiol. 48(4), 747–750.

    CAS  Google Scholar 

  139. Cirigliano, M. C. and Carman, G. M. (1985), Appl. Environ. Microbiol. 50(4), 846–850.

    CAS  Google Scholar 

  140. Chmiel, O., Traitler, H., Bauer, W., and Hammes, W. P. (1994), Food Biotechnol. 8(1), 7–33.

    Article  CAS  Google Scholar 

  141. Chmiel, O., Traitler, H., Hammes, W. P., and Bauer, W. (1994), Food Biotechnol. 8(1), 35–56.

    CAS  Google Scholar 

  142. Stuer, W., Jaeger, K. E., and Winkler, U. K. (1986), J. Bacteriol. 168(3), 1070–1074.

    CAS  Google Scholar 

  143. Sabuquillo, P., Reina, J., Fernández, G., Guisán, J. M., and Fernández, R. (1998), Biochim. Biophys. Acta 1388(2), 337–348.

    CAS  Google Scholar 

  144. Bastida, A., Sabuquillo, P., Armisen, P., Fernández, R., Huguet, J., and Guisán, J. M. (1998), Biotechnol. Bioeng. 58(5), 486–493.

    Article  CAS  Google Scholar 

  145. De la Casa, R. M., Sánchez-Montero, J. M., and Sinisterra, J. V. (1999), Biotechnol. Lett. 21(2), 123–128.

    Article  Google Scholar 

  146. Rua, M. L. and Ballesteros, A. (1994), Biotechnol. Tech. 8(1), 21–26.

    Article  CAS  Google Scholar 

  147. Kawaguchi, Y., Honda, H., Taniguchi-Morimura, J., and Iwasaki, S. (1989), Nature 341(6238), 164–166.

    Article  CAS  Google Scholar 

  148. Fusetti, F., Brocca, S., Porro, D., and Lotti, M. (1996), Biotechnol. Lett. 18(3), 281–286.

    Article  CAS  Google Scholar 

  149. Alberghina, L. and Lotti, M. (1996), in Engineering of/with Lipases, Malcata, F. X., ed., Kluwer Academic, Dordrecht, The Netherlands, pp. 219–228.

    Google Scholar 

  150. Brocca, S., Schmidt-Dannert, C., Lotti, M., Alberghina, L., and Schmid, R. D. (1998), Protein Sci. 7(6), 1415–1422.

    Article  CAS  Google Scholar 

  151. Brocca, S., Schmidt-Dannert, C., Lotti, M., Alberghina, L., and Schmid, R. D. (1999), Patent WO9914338A1.

  152. Brocca, S., Schmidt-Dannert, C., Lotti, M., Alberghina, L., and Schmid, R. D. (2000), Patent EP1012301A1.

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Authors and Affiliations

  1. Department d’Enginyeria Química, E.T.S.E., Universitat Autònoma de Barcelona, 08193, Bellaterra (Barcelona), Spain

    Pau Ferrer, José Luis Montesinos, Francisco Valero & Carles Solà

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  1. Pau Ferrer
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  2. José Luis Montesinos
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  3. Francisco Valero
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  4. Carles Solà
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Correspondence to Francisco Valero.

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Ferrer, P., Montesinos, J.L., Valero, F. et al. Production of native and recombinant lipases by Candida rugosa . Appl Biochem Biotechnol 95, 221–255 (2001). https://doi.org/10.1385/ABAB:95:3:221

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  • DOI: https://doi.org/10.1385/ABAB:95:3:221

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