Abstract
The effect of Cibacron Blue 3GA (CB) on the fluorescence emission spectra of bovine serum albumin (BSA) was investigated in cationic reversed micelles formed with cetyltrimethylammonium bromide (CTAB) compared with that in aqueous phase. The anionic CB had electrostatic interactions with cationic CTAB and affinity interactions with BSA in the reversed micelles. The addition of CB in the reversed micellar phase led to a great decrease in the fluorescence intensity of BSA and a remarkable red shift of the wavelength of emission maximum (λmax). The fluorescence intensity of BSA decreased and the λmax decreased 5 nm (blue shift) without the addition of CB in the reversed micellar phase. The fluorescence intensity of BSA with the addition of CB had the strongest value in the aqueous phase with the presence of CTAB, a less strong value in the reversed micellar phase, and a weak value in the aqueous phase without the presence of CTAB. The increase in λmax of BSA with the addition of CB in the reversed micellar phase might indicate the decrease in the hydrophobic microenvironment of the Trp residue of BSA, contrary to those microenvironments in the absence of CB.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Luisi, P. L. and Magid, L. J. (1986), CRC Crit. Rev. Biochem. 20, 409–421.
Leodidis, E. B. and Hatton, T. A. (1990), J. Phys. Chem. 94, 6412–6420.
Martinek, K., Levashov, A. V., Klyachko, N. L., Khmelnitsky, Y. I., and Berezin, I. V. (1986), Eur. J. Biochem. 155, 453–459.
Luisi, P. L., Giomini, M., Pileni, M. P., and Robinson, B. H. (1988), Biochim. Biophys. Acta 947, 209–249.
Skagerlind, P. and Holmberg, K. J. (1994), Dispers. Sci. Technol. 15, 317–323.
Bommarius, A. S., Hatton, T. A., and Wang, D. I. C. (1995), J. Am. Chem. Soc. 117, 4515–4523.
Nicot, C. and Waks, M. (1995), in Biotechnology and Genetic Engineering Reviews, vol. 13, Tombs, M. P., ed., Intercept, England, pp. 266–302.
Walde, P., Peng, Q., Fadnavis, N. W., Battistel, E., and Luisi, P. L. (1988), Eur. J. Biochem. 173, 401–409.
Nicot, C., Vacher, M., Denoroy, L., Kahn, P. C., and Waks, M. (1993), J. Neurochem. 60, 1283–1289.
Groome, N. P., Vacher, M., Nicot, C., and Waks, M. (1990), Biochem. Int. 21, 1–7.
Dekker, M., Hilhorst, R., and Laane, C. (1989), Anal. Biochem. 178, 217–226.
Hatton, T. A. (1989), in Surfactant-Based Separation Processes, Scamehorn, J. F. and Harwell, J. H., eds., Marcel Dekker, New York, pp. 55–90.
Luisi, P. L., Giomini, M., Pileni, M. P., and Robinson, B. H. (1988), Biochim. Biophys. Acta 947, 209–246.
Woll, J. M., Hatton, T. A., and Yamush, M. L. (1989), Biotechnol. Prog. 5, 57–62.
Coughlin, R. W. and Baclaski, J. B. (1990), Biotechnol. Prog. 6, 307–309.
Paradkar, V. M. and Dordick, J. S. (1991), Biotechnol. Prog. 7, 330–334.
Kelley, B. D., Wang, D. I. C., and Hatton, T. A. (1993), Biotechnol. Bioeng. 42, 1199–1208.
Kelley, B. D., Wang, D. I. C., and Hatton, T. A. (1993), Biotechnol. Bioeng. 42, 1209–1217.
Adachi, M., Yamazaki, M., Harada, M., Shioi, A., and Katch, S. (1997), Biotechnol. Bioeng. 53, 406–408.
Adachi, M., Shibata, K., Shioi, A., Harada, M., and Katoh, S. (1998), Biotechnol. Bioeng. 58, 649–653.
Sun, Y., Ichikawa, S., Sugiura, S., and Furusaki, S. (1998), Biotechnol. Bioeng. 58, 58–64.
Sun, Y., Gu, L., Tong, X. D., Bai, S., Ichikawa, S., and Furusaki, S. (1999), Biotechnol. Prog. 15, 506–512.
Zhang, T. X., Liu, H. Z., and Chen, J. Y. (1999), Biotechnol. Prog. 15, 1078–1082.
Vijayalakshmi, M. A. and Bertrand, O. (1989), Protein-Dye Interactions: Developments and Applications, Elsevier Applied Science, London.
Ramírez-Vick, J. E. and García, A. A. (1996–1997), Sep. Puri. Methods 25, 85–129.
Zhang, T. X., Liu, H. Z., and Chen, J. Y. (1999), Colloids Surf. A 162, 259–264.
Karukstis, K. K., Frazier, A. A., Loftus, C. T., and Tuan, A. S. (1998), J. Phys. Chem. B 103, 8163–8170.
Hof, M., Lianos, P., and Laschewsky, A. (1997), Langmuir 13, 2181–2183.
Lundgren, J. S., Heitz, M. P., and Bright, F. V. (1995), Anal. Chem. 67, 3775–3781.
Marzola, P. and Gratton, E. (1991), J. Phys. Chem. 95, 9488–9495.
Lakowicz, J. R. (1983), Principles of Fluorescence Spectroscopy, Plenum, New York.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Zhang, TX., Liu, HZ. & Chen, JY. Fluorescence investigation of affinity interaction between bovine serum albumin and triazine dye in reversed micelles. Appl Biochem Biotechnol 95, 163–174 (2001). https://doi.org/10.1385/ABAB:95:3:163
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1385/ABAB:95:3:163