Abstract
A yeast strain isolated in the laboratory from fruit juices was studied and classified as Candida sake. The strain produces an intracellular β-glucosidase when grown with cellobiose as the carbon source. The enzyme was purified by ion-exchange chromatography and gel filtration. The molecular mass of the purified intracellular β-glucosidase, estimated by gel filtration, was 240 kDa. The tetrameric structure of the β-glucosidase was determined following treatment of the purified enzyme with sodium dodecyl sulfate. The enzyme exhibited optimum activity at 52°C and pH 4.25 with citrate-phosphate buffer. The enzyme was active against soluble glycosides with the (1→4)-β configuration, and from Lineweaver Burk plots, a K m value of 6.9 mmol/L was found for p-nitrophenyl-β-d-glucopyranoside. The β-glucosidase was found to be tolerant to glucose inhibition with a K i value of 0.2 mol/L.
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Gueguen, Y., Chemardin, P. & Arnaud, A. Purification and characterization of an intracellular β-glucosidase from a Candida sake strain isolated from fruit juices. Appl Biochem Biotechnol 95, 151–162 (2001). https://doi.org/10.1385/ABAB:95:3:151
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DOI: https://doi.org/10.1385/ABAB:95:3:151