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Bioemulsifier production in batch culture using glucose as carbon source by Candida lipolytica

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Abstract

The yeast Candida lipolytica IA 1055 produced an inducible extracellular emulsification activity while utilizing glucose at different concentrations as carbon source during batch fermentation at 27°C. In all glucose concentrations studied, maximum production of emulsification activity was detected in the stationary phase of growth, after pH reached minimal values. The bioemulsifier isolated was a complex biopolymer constituting proteins, carbohydrates, and lipids. The results obtained in this work show that the biosynthesis of a bioemulsifier is not simply a prerequisite for the degradation of extracellular hydrocarbon.

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Authors and Affiliations

  1. Departamento de Química, NPCIAMB-UNICAP Rua Nunes Machado, 42, bloco J, Térreo CEP, 50050-590, Recife, PE, Brazil

    Leonie A. Sarubbo & Galba Maria Campos-Takaki

  2. Fundação de Saúde Amauri de Medeiros-FUSAM, Recife, PE, Brazil

    Maria do Carmo Marçal

  3. Laboratório de Imunopatologia Keizo Asami-LIKA, Brazil

    Maria Luisa C. Neves, Maria da Paz C. Silva & Lúcia F. Porto

  4. Departamento de Bioquímica, UFPE, Recife, PE, Brazil

    Maria da Paz C. Silva

  5. Departamento de Morfologia e Fisiologia Animal, UFRPE, Recife, PE, Brazil

    Lúcia F. Porto

Authors
  1. Leonie A. Sarubbo
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  2. Maria do Carmo Marçal
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  3. Maria Luisa C. Neves
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  4. Maria da Paz C. Silva
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  5. Lúcia F. Porto
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  6. Galba Maria Campos-Takaki
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Corresponding author

Correspondence to Galba Maria Campos-Takaki.

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Sarubbo, L.A., Marçal, M.d.C., Neves, M.L.C. et al. Bioemulsifier production in batch culture using glucose as carbon source by Candida lipolytica . Appl Biochem Biotechnol 95, 59–67 (2001). https://doi.org/10.1385/ABAB:95:1:59

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  • DOI: https://doi.org/10.1385/ABAB:95:1:59

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