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Applied Biochemistry and Biotechnology

, Volume 91, Issue 1–9, pp 405–411 | Cite as

Effect of a natural contaminant on foam fractionation of bromelain

  • Samuel Ko
  • Justin Cherry
  • Aleŝ Prokop
  • Robert D. Tanner
Article

Abstract

Foam fractionation is a simple, inexpensive method for separating and purifying proteins. Typically, a dilute bromelain solution with a pH ranging from 2.0 to 7.0 foams very well when bubbles are introduced into a foam fractionation column. It was observed, however, that the dilute enzyme solution only foamed between approximately pH 2.0 and 3.0 when the inner wall of the fractionation column was coated with a natural contaminant (okra residue). We studied the separation ratio and the protein mass recovery to explore the effect of a natural antifoaming agent on the foam fractionation of a dilute bromelain solution. The control variables used in this process were the initial bulk solution pH, which ranged from 2.0 to 7.0, and the superficial air velocity, which varied between 1.7 and 6.2 cm/s.

Index Entries

Bromelain foam fractionation protein protein separation protease recovery antifoaming agent natural antifoaming agent 

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References

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Copyright information

© Humana Press Inc 2001

Authors and Affiliations

  • Samuel Ko
    • 1
  • Justin Cherry
    • 1
  • Aleŝ Prokop
    • 1
  • Robert D. Tanner
    • 1
  1. 1.Chemical Engineering DepartmentVanderbilt UniversityNashville

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