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Applied Biochemistry and Biotechnology

, Volume 90, Issue 3, pp 261–272 | Cite as

Phosphodiesterase production in an aqueous two-phase system by Nicotiana tabacum 1507

  • Mladenka IlievaEmail author
  • Atanas Pavlov
  • Anastasia Bacalova
Article

Abstract

Studies were conducted on the production of phosphodiesterases by Nicotiana tabacum 1507 cell suspension in an aqueous two-phase system formed by adding 4% polyethylene glycol (mol wt 20,000) and 7.5% dextran (mol wt 70,000) to the medium. The time course of growth, biosynthesis, secretion, and partitioning of phosphodiesterases was followed in comparison with N. tabacum 1507 cultivation as a free suspension. Partitioning of phosphodiesterases took place mainly in the bottom dextran phase, and a possibility was revealed for obtaining an enzyme preparation with high phosphodiesterase activity.

Index Entries

Aqueous two-phase system Nicotiana tabacum 1507 phosphodiesterases plant cell suspension culture 

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Copyright information

© Humana Press Inc. 2001

Authors and Affiliations

  • Mladenka Ilieva
    • 1
    Email author
  • Atanas Pavlov
    • 2
  • Anastasia Bacalova
    • 2
  1. 1.Institute of Microbiology, Laboratory of Biotechnology and Industrial MicrobiologyBulgarian Academy of SciencesPlovdivBulgaria
  2. 2.Bulgarian Academy of SciencesInstitute of Molecular BiologySofiaBulgaria

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