Applied Biochemistry and Biotechnology

, Volume 90, Issue 3, pp 221–232 | Cite as

Conversion of ammonia to dinitrogen in wastewater by Nitrosomonas europaea

  • Niranjan Kumar Shrestha
  • Shigeru Hadano
  • Toshiaki Kamachi
  • Ichiro OkuraEmail author


Because Nitrosomonas europaea contains ammonia-oxidizing enzyme, nitrite reductase, and nitrous oxide reductase, the conversion of ammonia to dinitrogen was tried with different reaction conditions. In aerobic reaction conditions, ammonium was converted to nitrite (NO 2 ), while under oxygen-limiting or oxygen-free conditions, NO 2 -N formed from ammonia oxidation by N. europaea was reduced to N2O and dinitrogen with 22% conversion. During denitrification, optimal pH for the production of N2O and dinitrogen was found to be 7.0–8.0. Dinitrogen was not produced in acidic pH<7.0. A low partial oxygen pressure as well as oxygen-free conditions are favorable for high production of dinitrogen.

Index Entries

Denitrification dinitrogen nitrous oxide nitrification Nitrosomonas europaea 


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Copyright information

© Humana Press Inc. 2001

Authors and Affiliations

  • Niranjan Kumar Shrestha
    • 1
  • Shigeru Hadano
    • 2
  • Toshiaki Kamachi
    • 1
  • Ichiro Okura
    • 1
    Email author
  1. 1.Department of BioengineeringTokyo Institute of TechnologyYokohamaJapan
  2. 2.Environmental Systems Division, Environmental & Agricultural PlantEngineering & Construction, Toshiba Plant KensetsuTokyoJapan

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