Affinity purification of secreted alkaline phosphatase produced by baculovirus expression vector system
- 61 Downloads
Human secreted alkaline phosphatase (SEAP) was produced in a stablytransformed Spodoptera frugiperda Sf-9 insect cell line (Sfb4GalT) following infection with a recombinant Autographa californica multiple nuclear polyhedrovirus containing the SEAP gene under control of the polyhedrin promoter. An affinity chromatographic column prepared by linking 4-aminobenzylphosphonic acid to histidyl-expoxy-Sepharose was used to isolate SEAP from the cell supernatant following removal of cells and virus and 10-fold concentration through ultrafiltration. We found that the binding of SEAP on the affinity matrix follows the Langmuir isotherm model. In addition, either recycling SEAP sample through the column for 24 h or loading high SEAP concentrations resulted in a high-purity product. Some nonspecific binding of protein on the matrix occurred when low concentrations of SEAP sample were loaded. Finally, we found that SEAP binding occurs rapidly, i.e., within 30 min of adding the SEAP sample to the affinity matrix.
Index EntriesAffinity chromatography human secreted alkaline phosphatase baculovirus expression vector system insect cells recombinant protein protein purification
Unable to display preview. Download preview PDF.
- 1.Luckow, V. A. (1995), in Baculovirus Expression Systems and Biopesticides, Shuler, M. L., Wood, H. A., Granados, R. R., and Hammer, H. A., eds., Wiley-Liss, New York, pp. 51–90.Google Scholar
- 2.Marz, L., Altmann, F., Staudacher, E., and Kubelka, V. (1995), in Glycoprotein, vol. 29a, Montreuil, J., Vliegenthart, J. F. G., and Schachter, H., eds., Elsevier, Amsterdam, pp. 543–563.Google Scholar
- 3.Jarvis, D. L. (1997), in The Baculoviruses, Miller, L. K., ed., Plenum, New York, pp. 389–431.Google Scholar
- 7.Millan, J. L. (1986), J. Biol. Chem. 261, 3112–3115.Google Scholar
- 8.Endo, T., Ohbayashi, H., Hayashi, Y., Ikehara, Y., Kochibe, N., and Kobata, A. (1988), J. Biochem. 103, 182–187.Google Scholar
- 9.Sofer, G. K. and Nystron, L. E. (1989), Process Chromatography, A Practical Guide, Academic, London.Google Scholar
- 13.Vijayalakshmi, M. A. (1996), Mol. Biotechnol. 6, 347–357.Google Scholar
- 14.Matsumoto, I., Mizuno, Y., and Seno, N. (1979), J. Biochem. 85, 1091–1098.Google Scholar