Skip to main content
Log in

One- and two-stage upflow anaerobic sludge-bed reactor pretreatment of winery wastewater at 4–10°C

  • Published:
Applied Biochemistry and Biotechnology Aims and scope Submit manuscript

Abstract

The operating performance of a single and two (in series) laboratory upflow anaerobic sludge-bed (UASB) reactors (2.7-L working volume, recycle ratio varied from 1:1 to 1:18) treating diluted wine vinasse was investigated under psychrophilic conditions (4–10°C). For a single UASB reactor seeded with granular sludge, the average organic loading rates (OLRs) applied were 4.7, 3.7, and 1.7 g of chemical oxygen demand (COD)/(L·d) (hydraulic retention times [HRTs] were about 1 d) at 9–11, 6 to 7, and 4 to 5°C, respectively. The average total COD removal for preacidified vinasse wastewater was about 60% for all the temperature regimes tested. For two UASB reactors in series, the average total COD removal for treatment of non-preacidified wastewater exceeded 70% (the average OLRs for a whole system were 2.2, 1.8, and 1.3 g of COD/[L·d] under HRTs of 2 d at 10, 7, and 4°C, respectively). In situ determinations of kinetic sludge characteristics (apparent V m and K m ) revealed the existence of substantial mass transfer limitations for the soluble substrates inside the reactor sludge bed. Therefore, application of higher recycle ratios is essential for enhancement of UASB pretreatment under psychrophilic conditions. The produced anaerobic effluents were shown to be efficiently posttreated aerobically: final effluent COD concentrations were about 0.1 g/L. Successful operation of the UASB reactors at quite low temperatures (4–10°C) opens some perspectives for application of high-rate anaerobic pretreatment at ambient temperatures.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Kalyuzhnyi, S. V., Gladchenko, M. A., Sklyar, V. I., Kurakova, O. V., and Shcherbakov, S. S. (2000), Environ. Technol. 21, 919–925.

    Article  CAS  Google Scholar 

  2. Kalyuzhnyi, S. V., Sklyar, V. I., Davlyatshina, M. A., Parshina, S. N., Simankova, M. V., Kostrikina, N. A., and Nozhevnikova, A. N. (1996), Bioresour. Technol. 55, 47–54.

    Article  CAS  Google Scholar 

  3. Lurie, Y. Y. (1984), Analytical Chemistry of Industrial Wastewater, Khimiya, Moscow.

    Google Scholar 

  4. APHA. (1985), Standard Methods of the Examination of Water and Wastewater, 15th ed., American Health Association, Washington, DC.

    Google Scholar 

  5. Rebac, S., Gerbens, S., Lens, P., van Lier, J. B., Stams, A. J. M., Keesman, K. J., and Lettinga, G. (1999), Bioresour. Technol. 69, 241–248.

    Article  CAS  Google Scholar 

  6. Rebac, S. (1998), PhD thesis, Wageningen Agricultural University, The Netherlands.

    Google Scholar 

  7. Kato, M. T., Rebac, S., and Lettinga G. (1999), Appl. Biochem. Biotechnol. 76, 15–32.

    Article  CAS  Google Scholar 

  8. De Man, A. W. A., van der Last, A. R. M., and Lettinga, G. (1988), in Proceedings of the Fifth International Symposium on Anaerobic Digestion, Hall, E. R. and Hobson, P. N., eds., Pergamon, Oxford, England, pp. 197–209.

    Google Scholar 

  9. Vavilin, V. A., Vasiliev, V. B., and Rytov, S. V. (1990), Modeling of Destruction of Organic Matter by Microbial Community, Nauka, Moscow.

    Google Scholar 

  10. De Bazua, C. D., Cabrero, M. A., and Poggi, H. M. (1991), Bioresour. Technol. 35, 87–93.

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Sergey V. Kalyuzhnyi.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kalyuzhnyi, S.V., Gladchenko, M.A., Sklyar, V.I. et al. One- and two-stage upflow anaerobic sludge-bed reactor pretreatment of winery wastewater at 4–10°C. Appl Biochem Biotechnol 90, 107–124 (2001). https://doi.org/10.1385/ABAB:90:2:107

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1385/ABAB:90:2:107

Index Entries

Navigation