Applied Biochemistry and Biotechnology

, Volume 89, Issue 2–3, pp 171–181 | Cite as

Biosensor for determination of glucose and sucrose in fruit juices by flow injection analysis

  • Yann Guémas
  • Mohamed Boujtita
  • Nabil El Murr


Glucose and sucrose were measured with an amperometric method by using the flow injection analysis technique. A carbon paste electrode with a renewable surface containing glucose oxidase, horseradish peroxidase, and ferrocene was used in combination with the soluble enzymes invertase and mutarotase. The effect of invertase, mutarotase, and ascorbic acid on the electrode response was examined. Glucose and sucrose concentrations were determined with <3% errors. The proposed method for glucose and sucrose measurements was validated in real samples of fruitjuices. The results were also compared with those obtained with the ultraviolet method.

Index Entries

Biosensors food analysis glucose sucrose carbon paste electrode 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Satoh, I., Karube, I., and Suzuki, S. (1976), Biotechnol. Bioeng. 18, 269–272.PubMedCrossRefGoogle Scholar
  2. 2.
    Nabi Rahni, M. A., Lubrano, G. J., and Guilbault, G. G. (1987), J. Agric. Food Chem. 35, 1001–1004.CrossRefGoogle Scholar
  3. 3.
    Hamid, J. A., Moody, G. J., and Thomas, D. R. (1988), Analyst 113, 81–85.PubMedCrossRefGoogle Scholar
  4. 4.
    Barlíková, A., Svorc, J., and Miertus, S. (1991), Analytica Chimica Acta 247, 83–87.CrossRefGoogle Scholar
  5. 5.
    Neubauer, A., Hödl, C., Pum, D., and Sleytr, U. B. (1994), Analyt. Lett. 27(5), 849–865.Google Scholar
  6. 6.
    Salgado, A. M., Folly, R. O. M., Valdman, B., and Valero, F. (1998), Biotechnol. Techniques 12(4), 305–307.CrossRefGoogle Scholar
  7. 7.
    Scheller, F. and Renneberg, R. (1983), Analytica Chimica Acta 152, 265–269.CrossRefGoogle Scholar
  8. 8.
    Olsson, B., Stålbom, B., and Johansson, G. (1986), Analytica Chimica Acta 179, 203–208.CrossRefGoogle Scholar
  9. 9.
    Weise, H., Scheller, F., Kreibich, G., and Pfeiffer, D. (1981), Lebensmittelindustrie 28, 491–493.Google Scholar
  10. 10.
    Xu, Y. and Guilbault, G. G. (1989), Anal. Chem. 61, 782–784.PubMedCrossRefGoogle Scholar
  11. 11.
    Park, J.-K., Shin, M.-C., Lee, S.-G., and Kim, H.-S. (1995), Biotechnol. Prog. 11, 58–63.CrossRefGoogle Scholar
  12. 12.
    Matsumoto, K., Kamikado, H., Matsubara, H., and Osajima, Y. (1988), Anal. Chem. 60, 147–151.PubMedCrossRefGoogle Scholar
  13. 13.
    Matsumoto, K., Tsukatani, T., and Higuchi, S. (1995), Sensors Mater. 7(3), 167–177.Google Scholar
  14. 14.
    Masoom, M. and Townshend, A. (1985), Analytica Chimica Acta 171, 185–194.CrossRefGoogle Scholar
  15. 15.
    Masoom, M. and Townshend, A. (1985), Analyt. Proc. 22, 6–8.Google Scholar
  16. 16.
    Mizutani, F. and Asai, M. (1990), Analytica Chimica Acta 236, 245–250.CrossRefGoogle Scholar
  17. 17.
    Watanabe, E., Takagi, M., Takei, S., Hoshi, M., and Shu-gui, C. (1991), Biotechnol. Bioeng. 38, 99–103.CrossRefGoogle Scholar
  18. 18.
    Zhang, X. and Rechnitz, G. A. (1994), Electroanalysis 6, 361–367.CrossRefGoogle Scholar
  19. 19.
    Rondeau, A., Larson, N., Boujtita, M., Gorton, L., and El Murr, N. (1999), Analysis 27, 649–656.CrossRefGoogle Scholar
  20. 20.
    Boehringer Mannheim GmbH, Biochimica (1989), Methods of Biochemical Analysis and Food Analysis, Ed. Boehringer Mannheim, Mannheim, Germany.Google Scholar
  21. 21.
    Fung, Y.-S. and Mo, S.-Y. (1992), Analytica Chimica Acta 261, 375–380.CrossRefGoogle Scholar
  22. 22.
    Palmisano, F. and Zambonin, P. G. (1993), Anal. Chem. 65, 2690–2692.CrossRefGoogle Scholar
  23. 23.
    Miller, J. C. and Miller, J. N. (1993), Statistics for Analytical Chemistry, 3rd ed., E. Horwood PTR Prentice Hall, New York.Google Scholar

Copyright information

© Humana Press Inc. 2000

Authors and Affiliations

  • Yann Guémas
    • 1
  • Mohamed Boujtita
    • 1
  • Nabil El Murr
    • 1
  1. 1.Groupe Électrochimie—Laboratoire d’Analyse Isotopique et Électrochimique de Métabolismes (UPRSA-CNRS 6006)Faculté des Sciences et des TechniquesNantes Cedex 03France

Personalised recommendations