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Biosensor for determination of glucose and sucrose in fruit juices by flow injection analysis

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Abstract

Glucose and sucrose were measured with an amperometric method by using the flow injection analysis technique. A carbon paste electrode with a renewable surface containing glucose oxidase, horseradish peroxidase, and ferrocene was used in combination with the soluble enzymes invertase and mutarotase. The effect of invertase, mutarotase, and ascorbic acid on the electrode response was examined. Glucose and sucrose concentrations were determined with <3% errors. The proposed method for glucose and sucrose measurements was validated in real samples of fruitjuices. The results were also compared with those obtained with the ultraviolet method.

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References

  1. Satoh, I., Karube, I., and Suzuki, S. (1976), Biotechnol. Bioeng. 18, 269–272.

    Article  PubMed  CAS  Google Scholar 

  2. Nabi Rahni, M. A., Lubrano, G. J., and Guilbault, G. G. (1987), J. Agric. Food Chem. 35, 1001–1004.

    Article  Google Scholar 

  3. Hamid, J. A., Moody, G. J., and Thomas, D. R. (1988), Analyst 113, 81–85.

    Article  PubMed  Google Scholar 

  4. Barlíková, A., Svorc, J., and Miertus, S. (1991), Analytica Chimica Acta 247, 83–87.

    Article  Google Scholar 

  5. Neubauer, A., Hödl, C., Pum, D., and Sleytr, U. B. (1994), Analyt. Lett. 27(5), 849–865.

    CAS  Google Scholar 

  6. Salgado, A. M., Folly, R. O. M., Valdman, B., and Valero, F. (1998), Biotechnol. Techniques 12(4), 305–307.

    Article  CAS  Google Scholar 

  7. Scheller, F. and Renneberg, R. (1983), Analytica Chimica Acta 152, 265–269.

    Article  CAS  Google Scholar 

  8. Olsson, B., Stålbom, B., and Johansson, G. (1986), Analytica Chimica Acta 179, 203–208.

    Article  CAS  Google Scholar 

  9. Weise, H., Scheller, F., Kreibich, G., and Pfeiffer, D. (1981), Lebensmittelindustrie 28, 491–493.

    CAS  Google Scholar 

  10. Xu, Y. and Guilbault, G. G. (1989), Anal. Chem. 61, 782–784.

    Article  PubMed  CAS  Google Scholar 

  11. Park, J.-K., Shin, M.-C., Lee, S.-G., and Kim, H.-S. (1995), Biotechnol. Prog. 11, 58–63.

    Article  CAS  Google Scholar 

  12. Matsumoto, K., Kamikado, H., Matsubara, H., and Osajima, Y. (1988), Anal. Chem. 60, 147–151.

    Article  PubMed  CAS  Google Scholar 

  13. Matsumoto, K., Tsukatani, T., and Higuchi, S. (1995), Sensors Mater. 7(3), 167–177.

    CAS  Google Scholar 

  14. Masoom, M. and Townshend, A. (1985), Analytica Chimica Acta 171, 185–194.

    Article  CAS  Google Scholar 

  15. Masoom, M. and Townshend, A. (1985), Analyt. Proc. 22, 6–8.

    CAS  Google Scholar 

  16. Mizutani, F. and Asai, M. (1990), Analytica Chimica Acta 236, 245–250.

    Article  CAS  Google Scholar 

  17. Watanabe, E., Takagi, M., Takei, S., Hoshi, M., and Shu-gui, C. (1991), Biotechnol. Bioeng. 38, 99–103.

    Article  CAS  Google Scholar 

  18. Zhang, X. and Rechnitz, G. A. (1994), Electroanalysis 6, 361–367.

    Article  CAS  Google Scholar 

  19. Rondeau, A., Larson, N., Boujtita, M., Gorton, L., and El Murr, N. (1999), Analysis 27, 649–656.

    Article  CAS  Google Scholar 

  20. Boehringer Mannheim GmbH, Biochimica (1989), Methods of Biochemical Analysis and Food Analysis, Ed. Boehringer Mannheim, Mannheim, Germany.

    Google Scholar 

  21. Fung, Y.-S. and Mo, S.-Y. (1992), Analytica Chimica Acta 261, 375–380.

    Article  CAS  Google Scholar 

  22. Palmisano, F. and Zambonin, P. G. (1993), Anal. Chem. 65, 2690–2692.

    Article  CAS  Google Scholar 

  23. Miller, J. C. and Miller, J. N. (1993), Statistics for Analytical Chemistry, 3rd ed., E. Horwood PTR Prentice Hall, New York.

    Google Scholar 

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Authors and Affiliations

  1. Groupe Électrochimie—Laboratoire d’Analyse Isotopique et Électrochimique de Métabolismes (UPRSA-CNRS 6006), Faculté des Sciences et des Techniques, 2, rue de la Houssinière, B.P. 92208, 44322, Nantes Cedex 03, France

    Yann Guémas, Mohamed Boujtita & Nabil El Murr

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  1. Yann Guémas
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  2. Mohamed Boujtita
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  3. Nabil El Murr
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Guémas, Y., Boujtita, M. & El Murr, N. Biosensor for determination of glucose and sucrose in fruit juices by flow injection analysis. Appl Biochem Biotechnol 89, 171–181 (2000). https://doi.org/10.1385/ABAB:89:2-3:171

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  • DOI: https://doi.org/10.1385/ABAB:89:2-3:171

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