Biosensor for determination of glucose and sucrose in fruit juices by flow injection analysis
- 172 Downloads
Glucose and sucrose were measured with an amperometric method by using the flow injection analysis technique. A carbon paste electrode with a renewable surface containing glucose oxidase, horseradish peroxidase, and ferrocene was used in combination with the soluble enzymes invertase and mutarotase. The effect of invertase, mutarotase, and ascorbic acid on the electrode response was examined. Glucose and sucrose concentrations were determined with <3% errors. The proposed method for glucose and sucrose measurements was validated in real samples of fruitjuices. The results were also compared with those obtained with the ultraviolet method.
Index EntriesBiosensors food analysis glucose sucrose carbon paste electrode
Unable to display preview. Download preview PDF.
- 5.Neubauer, A., Hödl, C., Pum, D., and Sleytr, U. B. (1994), Analyt. Lett. 27(5), 849–865.Google Scholar
- 9.Weise, H., Scheller, F., Kreibich, G., and Pfeiffer, D. (1981), Lebensmittelindustrie 28, 491–493.Google Scholar
- 13.Matsumoto, K., Tsukatani, T., and Higuchi, S. (1995), Sensors Mater. 7(3), 167–177.Google Scholar
- 15.Masoom, M. and Townshend, A. (1985), Analyt. Proc. 22, 6–8.Google Scholar
- 20.Boehringer Mannheim GmbH, Biochimica (1989), Methods of Biochemical Analysis and Food Analysis, Ed. Boehringer Mannheim, Mannheim, Germany.Google Scholar
- 23.Miller, J. C. and Miller, J. N. (1993), Statistics for Analytical Chemistry, 3rd ed., E. Horwood PTR Prentice Hall, New York.Google Scholar