Abstract
A mediated modified carbon paste and renewable surface electrode for fructose amperometric measurement based on d-fructose dehydrogenase (FDH) was prepared and optimized. Commercially available ferrocene (FcH) and hydroxymethyl ferrocene (FcCH2OH) were used as mediators. The substituted FcH showed better linearity and higher sensitivity. The influence of different experimental parameters was studied for optimum analytical performance. The final FDH-modified electrode showed good analytical performance for batch mode measurements of fructose.
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Ikeda, T., Matsushita, F., and Senda, M. (1990), Agric. Biol. Chem. 54, 2919–2924.
Paredes, P. A., Parelleda, J., Fernàndez, V. M., Katakis, I., and Dominguez, E. (1997), Biosen. Bioelectron. 12, 1233–1243.
Garcia, C. A. B., De Olivia Neto, G., and Kuboto, L. T. (1998), Anal. Chem. Acta. 374, 201–208.
Matsumoto, K., Baeza, J. J., and Mottola, H. (1993), Anal. Chem. 65, 1658–1661.
Garcia, C. A. B., De Oliveira Neto, G., Kubota, L. T., and Grandin, L. A. J. (1996), Electroanal. Chem. 418, 147–151.
Matsumoto, K., Hamada, O., Ukeda, H., and Osajima, Y. (1998), Anal. Chem. 58, 2732–2374.
Kinnear, K. T. and Monbouquette, H. G. (1997), Anal. Chem. 69, 1771–1775.
Parellada, J., Dominguez, E., and Fernàndez, V. M. (1996), Anal. Chem. Acta. 330, 71–77.
Ikeda, T., Matsushita, F., and Senda, M. (1991), Biosen. Bioelectron. 6, 299–304.
Begum, A., Kobotake, E., Suzawa, T., Ikariyama, Y., and Aizawa, M. (1993), Anal. Chim. Acta 280, 31–36.
Khan, G. F., Kobotake, E., Shinohara, H., Ikariyama, Y., and Aizawa, M. (1992), Anal. Chem. 64, 1254–1258.
Khan, G. F., Shinihara, H., Ikariyama, Y., and Aizawa, M. (1991), J. Electroanal. Chem. 315, 263–273.
Gorton, L. (1995), Electroanalysis. 7, 23–45.
Mizutani, F. and Assai, M. (1991), Denki Kagaku (Tokyo) 58, 186, 187.
Khan, G., Kobotake, E., Ikariyama, Y., and Aizawa, M. (1993), Anal. Chim. Acta 281, 527–533.
El Murr, N. and Slilam, M. (1988), Patent Fr 88 05245.1988. (1990). Chem. Abstr. 112, 213,563.
Boujtita, M., Chapleau, M., and El Murr, N. (1996), Anal. Chim. Acta. 319, 91–96.
Boujtita, M., Chapleau, M., and El Murr, N. (1996), Electroanalysis 8, 485–488.
Chang, H., Ueno, A., Yamada, H., Matsue, T., and Ichida, I. (1991), Analyst 116, 793–796.
Urbaniczky, C. and Lundström, K. (1984), J. Electroanal. Chem. 176, 169–182.
Motta, N. and Guadalupe, A. H. (1994), Anal. Chem. 66, 566–571.
Narvàez, A., Parellada, J., Dominguez, E., and Katakis, I. (1996), Quimica Anal. 15, 75–82.
Ameyama, M., Shinagana, E., Matsushita, K., and Adachi, O. (1981), J. Bacteriol. 145, 814–823.
Yabuki, S. and Mizutani, F. (1997), Electroanalysis 9, 23–25.
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Boujtita, M., Murr, N.E. Ferrocene-mediated carbon paste electrode modified with d-fructose dehydrogenase for batch mode measurement of d-fructose. Appl Biochem Biotechnol 89, 55–66 (2000). https://doi.org/10.1385/ABAB:89:1:55
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DOI: https://doi.org/10.1385/ABAB:89:1:55