Water quality monitoring using an enhanced chemiluminescent assay based on peroxidase-catalyzed peroxidation of luminol

  • Anna D. Ilyina
  • José L. Martínez Hernández
  • Blanca H. López Luján
  • Juan E. Mauricio Benavides
  • Jorge Romero García
  • Jesús Rodríguez Martínez


Enhanced chemiluminescence (ECL) describes the phenomenon of the light output increase in the reaction of oxidation of luminol catalyzed by horseradish peroxidase (HRP) in the presence of certain phenolic compounds. This work summarizes the effects of preincubation of certain substances with HRP on the chemiluminescent reaction intensity. Preincubation of herbicide, detergent, surfactants (Brij-96 and Tween-20), phenol, metal ions (mercury, cobalt, and nickel), and bactericide with HRP had an inhibitory effect on the enzyme activity. HRP-preincubation with metal ions (cadmium, magnesium, and zinc), as well as with some insecticides, stimulated the chemiluminescent intensity. Calibration graphs were obtained to demonstrate the possibility to determine the pollutant concentration. Light emission from the peroxidase catalyzed enhanced chemilum inescence is affected by a wide number of chemicals and, therefore, the method can beused for on-site monitoring of water quality. A rapid and simple assay to detect water contamination has been developed.

Index Entries

Horseradish peroxidase luminol water pollutants 


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Copyright information

© Humana Press Inc. 2000

Authors and Affiliations

  • Anna D. Ilyina
    • 1
  • José L. Martínez Hernández
    • 1
  • Blanca H. López Luján
    • 1
  • Juan E. Mauricio Benavides
    • 1
  • Jorge Romero García
    • 2
  • Jesús Rodríguez Martínez
    • 1
  1. 1.Departamento de BiotecnologíaFacultad de Ciencias Químicas de la Universidad Autónoma de CoahuilaSaltilloMexico
  2. 2.Departamento de BiopolimerosCentro de Investigación en Química AplicadaMexico

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