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Oscillations of exopolymeric composition and sludge volume index in nitrifying flocs

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Abstract

Protein, carbohydrate, and lipid composition of the exopolymer fraction of a nitrifying sludge in steady-state culture was analyzed after dissociation with 50 mM EDTA and dialysis of the nonfilamentous flocs. Steady-state culture was established when the nitrification rate was constant. The nitrification efficiency at that regime was 93%, also constant. In steady state the concentration of exopolymer protein in the nitrifying sludge floc oscillated from 5 (lowest) to 45 (highest) mg/L with a consistent oscillating pattern having a duration period of 10 d each. Carbohydrate and lipid content in the flocs showed no significant variations (30 and 36 mg/L, respectively). Only 20% of the extracellular polysaccharides had molecular weights higher than 10 kDa, suggesting that the floc aggregation depended on smaller fractions of low-molecular-weight carbohydrates. The oscillations in the concentration of exopolymeric protein coincided with parallel variations in the sludge volumetric index (SVI) value (12.2±2.1 mL/g). Analysis of the polymeric substances of the floc and suspended solids corroborated by statistical analysis indicated that the variations in the SVI of the nitrifying nonfilamentous flocs were mainly related to the changes in the exopolymeric protein content.

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References

  1. Prosser, J. I. (1989), Adv. Microb. Physiol. 30, 125–181.

    CAS  Google Scholar 

  2. Böck, E., Koops, H. P., Harms, H., and Ahlers, B. (1991), in Variations in Autorophic Life, Böck, E., ed., Academic, London, pp. 171–200.

    Google Scholar 

  3. Watson, S. W., Böck, E., Harms, H., Koops, H. P., and Hooper, A. B. (1989), in Bergy’s Manual of Systematic Bacteriology, vol. 3, Stanly, J. T., Brynt, M., Pfennig, N., and Holt, J. G., eds., Williams & Wilkins, Baltimore, pp. 1807–1834.

    Google Scholar 

  4. Michael, G., Shimizu, G. P., and Jenkins, D. (1985), J. Water Pollut. Control Fed. 57, 1152–1162.

    Google Scholar 

  5. Urbain, V., Block, J. C., and Manem, J. (1993), Water Res. 27, 829–838.

    Article  CAS  Google Scholar 

  6. Jahn, A. and Nielsen, P. H. (1998), Water Sci. Technol. 37, 17–24.

    Article  CAS  Google Scholar 

  7. Chaw, A. C. and Keinath, T. M. (1979), Water Res. 13, 1213–1223.

    Article  Google Scholar 

  8. Magnusson, K. E. (1980), Scand. J. Infect. Dis. 24, 131–134.

    Google Scholar 

  9. Goodwin, J. A. S. and Forster, C. F. (1985), Water Res. 19, 527–533.

    Article  CAS  Google Scholar 

  10. Forster, C. F. (1985), Water Res. 19, 1259–1264.

    Article  CAS  Google Scholar 

  11. Sato, T. and Ose, Y. (1980), Water Res. 14, 333–338.

    Article  CAS  Google Scholar 

  12. Horan, N. J. and Eccles, C. R. (1986), Water Res. 20, 1427–1432.

    Article  CAS  Google Scholar 

  13. Eriksson, L. and Alm, B. (1991), Water Sci. Technol. 24, 21–28.

    CAS  Google Scholar 

  14. Jahn, A. and Nielsen, P. H. (1995), Water Res. 32(8), 157–164.

    CAS  Google Scholar 

  15. Sutherland, I. W. (1977), Am. Chem. Soc. Symp. Ser. 45, 40–56.

    CAS  Google Scholar 

  16. Forster, C. F. and Dallas-Newton, J. (1980), J. Water Pollut. Control Fed. 79, 338–351.

    CAS  Google Scholar 

  17. Jorand, F., Zartarian, F., Thomas, F., Block, J. C., Bottero, J. Y., Villemin, G., Urbain, V., and Manem, J. (1995), Water Res. 29, 1639–1647.

    Article  CAS  Google Scholar 

  18. Nielsen, P. H., Jahn, A., and Palmgren, R. (1997), Water Sci. Technol. 36, 11–19.

    Article  CAS  Google Scholar 

  19. Novak, L., Larrea, L., Wanner, J., and Garcia-Heras, J. L. (1993), Water Res. 27, 1339–1346.

    Article  CAS  Google Scholar 

  20. van Leeuwen, J. (1988), Water S.A. 15, 127–132.

    Google Scholar 

  21. Jenkins, D. (1992), Water Sci. Technol. 26, 215–230.

    Google Scholar 

  22. Foot, R. J., Robinson, M. S., and Forster, C. F. (1994), Water Sci. Technol. 29, 213–220.

    CAS  Google Scholar 

  23. Rudling, L. and Solyom, P. (1974), Water Res. 8, 115–119.

    Article  CAS  Google Scholar 

  24. Lowry, O. H., Rosenbrouth, N. J., Farr, A. L., and Randall, R. J. (1951), J. Biol. Chem. 193, 265–275.

    CAS  Google Scholar 

  25. Dubois, M., Gilles, K. A., Hamilton, J. K., Roberts, P. A., and Smith, F. (1956), Anal. Chem. 28, 350–352.

    Article  CAS  Google Scholar 

  26. Frings, C. S. and Dunn, R. T. (1970), J. Clin. Pathol. 53, 89–91.

    CAS  Google Scholar 

  27. APHA. (1985), Standard Methods for Examination of Water and Wastewater, 16th ed. American Public Health Association, Washington, DC.

    Google Scholar 

  28. Gomez, J., Mendez, R., and Lema, J. M. (1966), J. Biochem. Biotechnol. 57/58, 869–876.

    Article  Google Scholar 

  29. Khan, A. R. and Forster, C. F. (1990), Enzyme Microb. Technol. 12, 788–793.

    Article  CAS  Google Scholar 

  30. Nue, T. R. (1996), Microbiol. Rev. 60, 151–166.

    Google Scholar 

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Authors and Affiliations

  1. Departmento de Biotecnología, Universidad Autónoma Metropolitana Iztapalalapa, Av. Michoacán y Purisima, Vicentina, C.P. 09340, Iztapalapa, México, D.F., Mexico

    Felipe Martinez, Ernesto Favela-Torres & Jorge Gomez

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  1. Felipe Martinez
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  2. Ernesto Favela-Torres
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  3. Jorge Gomez
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Martinez, F., Favela-Torres, E. & Gomez, J. Oscillations of exopolymeric composition and sludge volume index in nitrifying flocs. Appl Biochem Biotechnol 87, 177–188 (2000). https://doi.org/10.1385/ABAB:87:3:177

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  • DOI: https://doi.org/10.1385/ABAB:87:3:177

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