Applied Biochemistry and Biotechnology

, Volume 87, Issue 2, pp 73–80 | Cite as

Semicontinuous production of lipase by Acinetobacter radioresistens in presence of nonwoven fabric

  • Sheng-Chou Li
  • Jau-Yann Wu
  • Chuh-Yung Chen
  • Teh-Liang Chen


The effect of a hydrophobic nonwoven fabric on the lipase production by Acinetobacter radioresistens was investigated with semicontinuous culture. The fermentation medium contained n-hexadecane as the carbon source. The nonwoven fabric was made from nylon 6 and coated with an acrylic resin. Equipping the nonwoven fabric around the baffles of a 2.5-L agitated fermentor could provide a fine dispersion of n-hexadecane, thus enhancing lipase production. The improvement on lipase yield by using the nonwoven fabric was found to be comparable to that of using an emulsifier (gum arabic). Compared with the corresponding culture in the absence of nonwoven fabric, the employment of the nonwoven fabric could significantly enhance both lipase yield and volumetric productivity.

Index Entries

Lipase production Acinetobacter radioresistens semicontinuous culture nonwoven fabric dispersion of oil 


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Copyright information

© Humana Press Inc. 2000

Authors and Affiliations

  • Sheng-Chou Li
    • 1
  • Jau-Yann Wu
    • 2
  • Chuh-Yung Chen
    • 1
  • Teh-Liang Chen
    • 1
  1. 1.Department of Chemical EngineeringNational Cheng Kung UniversityTainanTaiwan
  2. 2.Department of Chemical EngineeringI-Shou University, Ta-Hsu Hsiang, Kaohsiung CountyTaiwan

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