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Immobilized Streptomyces clavuligerus NP1 cells for biotransformation of penicillin G into deacetoxycephalosporin G

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Abstract

An investigation was conducted to determine whether immobilized resting cells of Streptomyces clavuligerus NP1, entrapped on a polymeric matrix, are able to perform oxidative ring expansion of benzylpenicillin into deacetoxycephalosporin G by virtue of their deacetoxycephalosporin C synthase (“expandase”) activity. Cells entrapped in polyethyleneimine-barium alginate (1.5%) were able to sustain activity for at least four 2-hcycles, whereas free resting cells were inactive after the second cycle. Although entrapped cells exhibited lower oxidative ring expansion activity than free resting cells, immobilization may offer storage stability, recyclability, and operational stability for biotransformation of penicillins to cephalosporins, thus contributing to the development of a biological means for the production of the important industrial intermediate 7-aminodeacetoxycephalosporanic acid.

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Author notes

  1. Marco A. Báez-Vásquez

    Present address: Centro de Biotecnología, Instituto Tecnológico y de Estudios Superiores de Monterrey-Campus Monterrey, Av. Eugenio Garza Sada Sur 2501, CP.64849, Monterrey, N.L., México

Authors and Affiliations

  1. Fermentation Microbiology Laboratory, Department of Biology, Massachusetts Institute of Technology, 02139, Cambridge, MA

    Arnold L. Demain & Marco A. Báez-Vásquez

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  1. Arnold L. Demain
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  2. Marco A. Báez-Vásquez
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Correspondence to Arnold L. Demain.

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Demain, A.L., Báez-Vásquez, M.A. Immobilized Streptomyces clavuligerus NP1 cells for biotransformation of penicillin G into deacetoxycephalosporin G. Appl Biochem Biotechnol 87, 135–140 (2000). https://doi.org/10.1385/ABAB:87:2:135

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  • DOI: https://doi.org/10.1385/ABAB:87:2:135

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