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Butanol production using Clostridium beijerinckii BA101 hyper-butanol producing mutant strain and recovery by pervaporation

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Abstract

Clostridium beijerinckii BA101 (mutant strain) and C. beijerinckii 8052 (wild type) were compared for substrate and butanol inhibition. The wild-type strain is more strongly inhibited by added butanol than is the mutant strain. Acetone and butanol were removed from a fed-batch reactor inoculated with C. beijcrinckii BA101 by pervaporation using a silicone membrane. In the batch reactor, C. beijerinckii BA101 produced 25.3 g/L of total solvents, whereas in the fermentation-recovery experiment it produced 165.1 g/L of total solvents. Solvent productivity increased from 0.35 (batch reactor) to 0.98 g/L·h (fed-batch reactor). The fed-batch reactor wasfed with 500 g/L of glucose-based P2 medium. Acetone selectivities ranged from 2 to 10 whereas butanol selectivities ranged from 7 to 19. Total flux varied from 26 to 31 g/m2·h.

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Abbreviations

A :

Pervaporation membrane area (m2)

C g :

Glucose concentration in concentrated feed medium (C g1 and C g2 for first and second additions, respectively) (g/L)

C g0 :

Glucose concentration in fermentation reactor at 0 time (g/L)

C ge :

Glucose concentration in fermentation broth at the end of fermentation (g/L)

G gn :

Glucose concentration in nth addition of concentrated feed medium (n=1,2,3,… n) (g/L)

C s :

Solvent concentration in condensate (C s1 and C s2 for first and second condensates, respectively) (g/L)

C af :

Solvent concentration in fermentation broth at the end of pervaporation (g/L)

C sn :

Solvent concentration in nth condensate (n=1,2,3, … n) (g/L)

H :

Time period during which pervaporation membrane condensate was collected (h)

R p :

Reactor/solvent productivity (g/L·h)

T :

Fermentation time (h)

V c :

Volume of pervaporation membrane condensate (V c1 and V c2 for first and second condensates, respectively) (L)

V cv :

Volume of nth condensate of pervaporation membrane (n=1,2,3,… n) (L)

V fD :

Volume of fermentation broth at 0 time (L)

V fc :

Volume of fermentation broth at the end of fermentation (L)

V fc :

Volume of fermentation broth at the end of pervaporation (L)

V g :

Volume of concentrated feed medium added to the fed-batch reactor (L)

V gn :

Volume of nth addition of concentrated feed medium to the fedbatch reactor (n=1,2,3,…n) (L)

W :

Weight of condensate collected from the pervaporation membrane (g)

X :

Weight fraction of butanol or total solventin fermentation broth(−)

Y :

Weight fraction of butanol or total solvent in the membrane permeate (condensate) (−)

Y p/B :

Yield of solvents (g solvents/g glucose utilized)

α:

Selectivity of acetone/butanol or total solvents (−)

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Authors and Affiliations

  1. University of Illinois, Biotechnology and Bioengineering Group, Department of Food Science and Human Nutrition, 1207 W. Gregory Drive, 61801, Urbana, IL

    N. Qureshi & H. P. Blaschek

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  1. N. Qureshi
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  2. H. P. Blaschek
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Correspondence to N. Qureshi.

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Qureshi, N., Blaschek, H.P. Butanol production using Clostridium beijerinckii BA101 hyper-butanol producing mutant strain and recovery by pervaporation. Appl Biochem Biotechnol 84, 225–235 (2000). https://doi.org/10.1385/ABAB:84-86:1-9:225

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  • DOI: https://doi.org/10.1385/ABAB:84-86:1-9:225

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