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Fermentations of pectin-rich biomass with recombinant bacteria to produce fuel ethanol

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Abstract

Pectin-rich residues from sugar beet processing contain significant carbohydrates and insignificant amounts of lignin. Beet pulp was evaluated for conversion toethanol using recombinant bacteria as biocatalysts. Hydrolysis of pectin-rich residues followed by ethanolic fermentations by yeasts has not been productive because galacturonic acid and arabinose are not ferm entable toethanol by these organisms. The three recombinant bacteria evaluated in this study, Escherichia coli strain KO11, Klebsiella oxytoca strain P2, and Erwinia chrysanthemi EC 16 pLOI 555, ferment carbohydrates in beet pulp with varying efficiencies. E. coli KO11 is able to convert pure galactu ronic acid to ethanol with minimal acetate production. Using an enzyme loading of 10.5 filter paper un its of cellulase, 120.4 polygalactu ronase units of pectinase, and 6.4 g of cellobiase (per gram of dry wt sugar beet pulp), with substrate addition after 24 h of fermentation, 40 g of ethanol/L was produced. Other recombinants exhibited lower ethanol yields with increases in acetate and succinate production.

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References

  1. Hall, D. O. (1979), Sofar Energy 22, 307–328.

    Article  CAS  Google Scholar 

  2. Picataggio, S. K., Zhang, M., and Finkelstein, M. (1994), in Enzymatic Conversion of Biomass for Fuels Production, Himmel, M. E., Baker, J. O., and Overend, R. P., eds., ACS Symposium Series 566, American Chemical Society, Washington, DC pp. 342–371.

    Google Scholar 

  3. Grohmann, K. and Bothast, R. (1994), in Enzymatic Conversion of Biomass for Fuels Production, Himmel, M. E., Baker, J. O., and Overend, R. P., eds. ACS Symposium Series 566, pp. 373–390.

  4. Clarke, M. and Edye, L. (1996), in Agricultural materials as Reneuwable Resources, Fuller, G., McKeon, T. A., and Bills, D. D., eds. ACS Symposium Series 647, pp. 228–247.

  5. Nigam, P. (1994), Process Biochem. 29, 337–342.

    Article  CAS  Google Scholar 

  6. Coons, F. F. (1982), in Beet Sugar Technology, McGinnis, R. A., ed. Beet Sugar Development Foundation, Denver, CO, pp. 587–599.

    Google Scholar 

  7. Lynd, L. R., Cushman, J. H., Nichols, R. J. and Wyman, C. E. (1991), Science 251, 1318–1323.

    Article  CAS  Google Scholar 

  8. Dumsday, G., Jones, K., Stanley, G., and Pamment, N. B. (1997), Aust ralasian Biotechnol 7, 285–295.

    CAS  Google Scholar 

  9. Ingram, L. O., Lai, X., Moniruzzaman, M., Wood, B. E., and York, S. W. (1997), in Fuels and Chemicals from Biomass, Saha, B. C. and Woodward, J., eds. ACS Symposium Series 666, pp. 57–72.

  10. Krishan, M. S., Xia, Y., Ho, N. W. Y., and Tsao, G. T. (1997), in Fuels and Chemicals from Biomass, Saha, B. C. and Woodward, J., eds. ACS Symposium Series 666, pp. 74–92.

  11. Jeffries, T. W. (1990), in Yest Biotechnology and Biocatalysis, Verrachtert, H. and Demot, R., eds., Maroel Dekker, New York, pp. 349–394.

    Google Scholar 

  12. Michel, F., Thibault, J.-F., and Barry, J.-L. (1988), J. Sci. Food Agric. 42, 77–85.

    Article  CAS  Google Scholar 

  13. Zhang, M., Eddy, C., Deanda, K., Finkelstein, M., and Picataggio, S. (1995), Science, 267, 240–243.

    Article  CAS  Google Scholar 

  14. Deanda, K., Zhang, M., Eddy, C., and Picataggio S. (1996), Appl. Environ. Microbiol. 62, 4465–4470.

    CAS  Google Scholar 

  15. Ingram, L. O., Conway, T., Clark, D. P., Sewell, G. W., and Preston, J. F. (1987), Appl. Environ. Microbiol. 53, 2420–2425.

    CAS  Google Scholar 

  16. Wood, B. E. and Ingram, L. O. (1992), Appl. Environ. Microbiol. 58, 2103–2110.

    CAS  Google Scholar 

  17. Beall, D. S., and Ingram, L. O. (1993), I. Indust. Microbiol. 11, 151–155.

    Article  CAS  Google Scholar 

  18. Ingram, L. O. and Doran, J. B. (1994), FEMS Microbiol. Rev. 16, 235–241.

    Article  Google Scholar 

  19. Grohmann, K., Baldwin, E. A., Buslig, B. S., and Ingram, L. O. (1994). Biotechnol. Lett. 16, 281–286.

    Article  CAS  Google Scholar 

  20. Doran, J. B., Cripe, J. and Sutton, M. (1997), in American Society of Sugar Beet Technologists, Proceedings from the 29th Biennial Meeting, Beet Sugar Development Foundation, Denver, CO, pp. 235–238.

  21. Doran, J. B. and Ingram, L. O. (1993), Biotechnol. Prog. 9, 533–538.

    Article  CAS  Google Scholar 

  22. Brooks, T. A. and Ingram, L. O. (1995), Biotechnol. Prog. 11, 619–625.

    Article  CAS  Google Scholar 

  23. Doran, J. B., Aldrich, H. A., and Ingram L. O. (1994), Biotechnol. Bioeng. 44, 240–247

    Article  CAS  Google Scholar 

  24. Wang, C. C. H. and Chang, K. C. (1994), J. Food Sci. 59, 1165–1167.

    Google Scholar 

  25. Nelson, D. B., Smith, C. J. B., and Wiles, R. R. (1977), in Food Colloids, Grahm, H. D., ed., AVI Publishing, Westport, CT, pp. 418–430.

    Google Scholar 

  26. Aspinall, G. O. (1970) in The Carbohydrates, Chemistry and Biochemistry, Pigman, W. and Horton, D., eds., Academic, New York, pp. 515–536.

    Google Scholar 

  27. Micard, V., Renard, C. M. G. C., and Thibault, J. F. (1996), Enzyme Microb. Technol. 19, 162–170.

    Article  CAS  Google Scholar 

  28. Ingram, L. O., Conway, T., and Altherthum, F. (1991), Ethanol production by Escherichia coli strains co-expressing Zymomonas mobilis PDC and ADH genes, U. S. Patent No. 5,000,000.

  29. Ingram, L. O. and Conway, T. (1988), Appl. Environ. Microbiol. 54, 397–404.

    CAS  Google Scholar 

  30. Burchhardt, G. and Ingram, L. O. (1992), Appl. Environ. Microbiol. 58, 1128–1133.

    CAS  Google Scholar 

  31. Ohta, K., Beall, D., Mejia, J., Shanmugan, K., and Ingram, L. (1991), Appl. Environ. Microbiol. 57, 893–900.

    CAS  Google Scholar 

  32. Spagnuolo, M., Crecchio, C., Pizzigallo, M., and Ruggiero, P. (1997), Biorosour. Technol. 60, 215–222.

    Article  CAS  Google Scholar 

  33. Mandels, M., Andreotti, R., and Roche, C. (1976), Biotechnol. Bioeng. Symp. 6, 21–33.

    CAS  Google Scholar 

  34. Beall, D. S., Ohta, K., and Ingram, L. O. (1991) Biotechnol. Bioeng. 38, 296–303.

    Article  CAS  Google Scholar 

  35. Philippidis, G. P. (1994), in Enzymatic Conversion of Biomass for Fuels Production, Himmel, M. E., Baker, J. O., and Overend, R. P., eds. ACS Symposium Series 556, pp. 188–217.

  36. von Sivers, M. and Zacchi, G. (1995), Bioresour. Technol. 51, 43–52.

    Article  Google Scholar 

  37. Enari, T.-M. (1983), in Microbial Enzymes and Biotechnology, Fogarty, W., ed., Applied Science Publishers, New York, pp. 183–223.

    Google Scholar 

  38. Eriksson, K.-E. L., Blanchette, R. A., and Ander, P. (1990), Microbial and Enzymatic Degradation of Wood and Wood Components, Springer Verlag, New York.

    Google Scholar 

  39. Chahal, D. S. (1991), in Enzymes in Biomass Conversion, Leatham, G. and Himmel, M., eds, ACS Symposium Series 460, pp. 111–122.

  40. Gauss, W. F., Suzuki, S., and Takagi, M. (1976), Manufacture of alcohol from cellulosic materials using plural ferments. U.S. Patent No. 3,990,9.

  41. Eklund, R. and Zacchi, G. (1995), Enzyme Microb. Technol. 17, 255–259.

    Article  CAS  Google Scholar 

  42. Lawford, H. and Rousseau, J. (1995), Biotechnol. Lett. 17, 751–756.

    Article  CAS  Google Scholar 

  43. Lawford, H. and Rousseau, J. (1996), Appl. Biochem. Biotechnol. 57/58, 277–292.

    Article  CAS  Google Scholar 

  44. Dumsday, G., Zhou, B., Buhmann, S., Stanley, G. and Pamment, N. (1997), Australasian Biotechnol. 7, 300–303.

    CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Biology, Central Michigan University, Mt, 217 Brooks Hall, 48859, Pleasant, MI

    Joy Bethune Doran, Jennifer Cripe, Misty Sutton & Brian Foster

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  1. Joy Bethune Doran
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  2. Jennifer Cripe
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  3. Misty Sutton
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  4. Brian Foster
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Correspondence to Joy Bethune Doran.

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Doran, J.B., Cripe, J., Sutton, M. et al. Fermentations of pectin-rich biomass with recombinant bacteria to produce fuel ethanol. Appl Biochem Biotechnol 84, 141–152 (2000). https://doi.org/10.1385/ABAB:84-86:1-9:141

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  • DOI: https://doi.org/10.1385/ABAB:84-86:1-9:141

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