Applied Biochemistry and Biotechnology

, Volume 82, Issue 3, pp 175–183 | Cite as

Xanthan gum production by Xanthomonas campestris w.t. fermentation from chestnut extract

  • M. Liakopoulou-KyriakidesEmail author
  • S. K. Psomas
  • D. A. Kyriakidis


Xanthomonas campestris w.t. was used for production of xanthan gum in fermentations with chestnut flour for the first time. Fermentations were carried out with either chestnut flour or its soluble sugars (33.5%) and starch (53.6%), respectively, at 28°C and 200 rpm at initial pH 7.0 in flasks. The effect of agitation rate (at 200, 400, and 600 rpm) on xanthan gum production was also studied in a 2-L batch reactor. It was found that xanthan production reaches a maximum value of 3.3 g/100 mL at 600 rpm and 28°C at 45 h.

Index Entries

Xanthomonas campestris fermentation xanthan gum chestnuts 


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  1. 1.
    Moorhouse, R., Walkinshaw, D., and Arnott, S. (1977), in Extracellular Microbial Polysaccharides, ACS Symposium Series, American Chemical Society, Washington, DC, pp. 91–102.Google Scholar
  2. 2.
    Andrew, T. R. (1997), in Extracellular Microbial Polysaccharides, ACS Symposium Series, Sanford, P. A. and Laskin, A., eds., American Chemical Society, Washington, DC, pp. 231–241.Google Scholar
  3. 3.
    Jeans, A. (1974), J. Polymer Sci. Part C, Polymer Symp. 45, 209–227.CrossRefGoogle Scholar
  4. 4.
    De Vuyst, L. and Vermeire A. (1994), Appl. Microbiol. Biotechnol. 42, 187–191.Google Scholar
  5. 5.
    Papoutsopoulou, S. V., Ekateriniadou, L. V., and Kyriakidis, D. A. (1994), Biotechnol. Lett. 16, 1235–1240.Google Scholar
  6. 6.
    Fu, J. F., Chang, R. Y., and Tseng, Y. H. (1992), Appl. Microbiol. Biotechnol. 37, 225–229.CrossRefGoogle Scholar
  7. 7.
    Konicek, J. and Konickova-Radockova, M. (1992), Folia Microbiol. 37, 102–104.CrossRefGoogle Scholar
  8. 8.
    Thorne, L., Tansey, L., and Pollock, T. J. (1998), J. Ind. Microbiol. 5, 321–328.Google Scholar
  9. 9.
    Papi, R. M., Ekateriniadou, L. V., Beletsiotis, E., Typas, M. A., and Kyriakidis, D. A. (1999), Biotechnol. Lett. 21, 39–43.CrossRefGoogle Scholar
  10. 10.
    Maniatis, T., Fritsch, E.F., and Sambrook, J. (1989), Molecular Cloning: A Laboratory Manual, 2nd ed., Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.Google Scholar
  11. 11.
    Ekateriniadou, L. V., Papoutsopoulou, S. V., and Kyriakidis, D. A. (1994), Biotechnol. Lett. 16, 517–522.CrossRefGoogle Scholar
  12. 12.
    Miller, G. L. (1959), Anal. Chem. 31, 426–428.CrossRefGoogle Scholar
  13. 13.
    Dubois, M., Gilles, K. A., Hamilton, J. K., Rebers, P. A., and Smith, F. (1956), Anal. Chem. 28, 350–356.CrossRefGoogle Scholar
  14. 14.
    Bearden, J. C. (1978), Biochim. Biophys. Acta 533, 525–529.Google Scholar
  15. 15.
    Bradford, M. M. (1976), Ann. Biochem. 72, 248–254.CrossRefGoogle Scholar
  16. 16.
    American Association of Cereal Chemists (1983), Approved Methods of the American Association of Cereal Chemists, 8th ed., AACC, St. Paul, MN.Google Scholar
  17. 17.
    Liakopoulou-Kyriakides, M., Tzanakakis, E. S., Kyparissidis, C., Ekateriniadou, L. V., and Kyriakidis, D. A. (1997), Chem. Eng. Technol. 20, 354–360.CrossRefGoogle Scholar
  18. 18.
    Fu, J. F. and Tseng, Y. H. (1990), Appl. Environ. Microbiol. 56, 919–923.Google Scholar
  19. 19.
    Carlos Roseiro, J., Nick Emery, A., Simoes, P., Estevao, F., and Amaral-Collaco, M. T. (1993), Appl. Microbiol. Biotechnol. 38, 709–713.CrossRefGoogle Scholar
  20. 20.
    Cadmus, M. C., Knutson, C. A., Lagoda, A. A., Pittsley, J. E., and Burton, K. A. (1978), Biotechnol. Bioeng. 20, 1003–1014.CrossRefGoogle Scholar
  21. 21.
    Davidson, I. W. (1978), FEMS Microbiol. Lett. 3, 347–349.CrossRefGoogle Scholar
  22. 22.
    Sutherland, I. W. (1981), Carbohydr. Polym. 1, 107–115.CrossRefGoogle Scholar
  23. 23.
    Peters, H.-U., Suh, I.-S., Schumpe, A., and Deckwer, W.-D. (1993), Biotechnol. Lett. 15, 565, 566.CrossRefGoogle Scholar

Copyright information

© Humana Press Inc. 1999

Authors and Affiliations

  • M. Liakopoulou-Kyriakides
    • 1
    Email author
  • S. K. Psomas
    • 1
  • D. A. Kyriakidis
    • 2
  1. 1.Department of Chemical EngineeringAristotle University of ThessalonikiThessalonikiGreece
  2. 2.Faculty of ChemistryAristotle University of ThessalonikiThessalonikiGreece

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