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Xylitol production from hardwood hemicellulose hydrolysates by Pachysolen tannophilus, Debaryomyces hansenii, and Candida guilliermondii

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Abstract

Three different yeasts, Pachysolen tannophilus, Debaryomyces hansenii, and Candida guilliermondii, were evaluated to ferment xylose solutions prepared from hardwood hemicellulose hydrolysates, among which P. tannophilus proved to be the most promising microorganism. However, the presence of both lignin-derived compounds (LDC) and acetic acid rendered a poor fermentation. To enhance the fermentation kinetics, different treatments to purify the hydrolysates were studied, including overliming, charcoal adsorption for LDC removal, and evaporation for acetic acid and furfural stripping. Under the best operating conditions assayed, 39.5g/L of xylitol were achieved after 96 h of fermentation, which corresponds to a volumetric productivity of 0.41 g/L·h and a yield of product on consumed substrate of 0.63 g p /gS.

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Authors and Affiliations

  1. Department of Chemical and Process Engineering G.B. Bonino, University of Genoa, Via Opera Pia 15, 16145, Genoa, Italy

    Attilio Converti & Patrizia Perego

  2. Department of Chemical Engineering, University of Vigo (Campus Ourense), Polytechnical Building, Las Lagunas, 32004, Ourense, Spain

    José Manuel Domínguez

Authors
  1. Attilio Converti
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  2. Patrizia Perego
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  3. José Manuel Domínguez
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Correspondence to José Manuel Domínguez.

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Converti, A., Perego, P. & Domínguez, J.M. Xylitol production from hardwood hemicellulose hydrolysates by Pachysolen tannophilus, Debaryomyces hansenii, and Candida guilliermondii . Appl Biochem Biotechnol 82, 141–151 (1999). https://doi.org/10.1385/ABAB:82:2:141

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  • DOI: https://doi.org/10.1385/ABAB:82:2:141

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