Abstract
Two cationic peroxidases isolated from Vaccinium myrtillus were encapsulated in reverse micelles of bis(2-ethylhexyl)sodium sulfosuccinate/isooctane. By using a central composite design, some relevant parameters for the enzymatic activity, such as surfactant and water concentration, pH, and buffer molarity, were analyzed. With the results obtained from this experimental planning, the response surface curves were established. The maximum specific activity obtained (0.19 mM/min · mM of enzyme) was approximately the same for both peroxidases, but the experimental conditions under which this value was attained differed considerably.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Welinder, K. G. (1992), Curr. Opin. Struc. Biol. 2, 388–393.
Grambow, H. J. (1986), in Molecular and Physiological Aspects of Plant Peroxidases, Greppin, H., Penel, C., and Gaspar, T., eds., University of Geneva, Switzerland, pp. 31–41.
Moerschbacher, B. M. (1992), in Plant Peroxidases 1980–1990, Greppin, H., Penel, C., and Gaspar, T., eds., University of Geneva, Switzerland, pp. 91–99.
Stinzie, A., Heitz, T., Prasad, V., Wiedemannmerdinoglu, S., Kauffman, S., Geoffroy, P., Legrand, M., and Fritig, B. (1993), Biochimie 75, 687–706.
Breda, C., Buffard, D., van Huystee, R. B., and Esnault, R. (1993), Plant Cell Rep. 12, 268–272.
Krell, H.-W. (1991), in Plant Peroxidases 1980–1990, Greppin, H., Penel, C., and Gaspar, T., eds., University of Geneva, Switzerland, pp. 469–478.
Urrutigoity, M. and Souppe, J. (1989), Biocatalysis 2, 145–149.
Wong, C.-H. and Whitesides, G. M. (1994), in Enzyme in Synthetic Organic Chemistry, Elsevier Science, Oxford, UK, pp. 131–194.
Yamada, Y., Kobayashi, S., Watanabe, K., and Hayashi, U. (1987), J. Chem. Tech. Biotechnol. 38, 31–39.
Arseguel, D., Lattes, A., and Baloulène, M. (1990), Biocatalysis 3, 227–233.
Metelitza, D. I., Eryomin, A. N., and Shibaev, V. A. (1992), Biocatalysis 5, 183–194.
Souppe, J., Urrutigoity, M., and Levesque, G. (1988), Biochim. Biophys. Acta 957, 254–257.
Gebicka, L. and Pawlak, J. (1997), J. Mol. Catal. B:Enzymatic 2, 185–192.
Setti, L., Fevereiro, P., Melo, E. P., Pifferi, P. G., Cabral, J. M. S., and Aires-Barros, M. R. (1995), Appl. Biochem. Biotechnol. 55, 207–218.
Rao, A. M., John, V. T., Gonzalez, R. D., Akkara, J. A., and Kaplan, D. L. (1993), Biotechnol. Bioeng. 41, 531–540.
Melo, N. S., Larsen, E., Welinder, K. G., and Fevereiro, M. P. (1997), Plant Sci. 122, 1–10.
Melo, N. S., Cabral, J. M. S., and Fevereiro, M. P. (1995), Plant Sci. 106, 177–184.
Barker, T. B. (1985), in Quality by Experimental Design, Marcel Dekker, New York.
Box, G. E. P., Hunter, W. G., and Hunter, J. S. (1985), in Statistics for Experimenters, John Wiley & Sons, New York.
Deming, S. N. and Morgan, S. L. (1987), in Science and Technology, vol. 3, Elsevier Science, Amsterdam.
Martinek, K., Klyachko, N. L., Kabanov, A. V., Khmelnitsky, Y. L., and Levashov, A. V. (1989), Biochim. Biophys. Acta 981, 161–172.
Serralheiro, M. L. and Cabral, J. M. S. (1999), Biocatal. Biotransform. 17, 3–19.
Laame, C., Hilhorst, R., and Veeger, C. (1987) in Methods in Enzymology, vol. 136, Mosbach, K., ed., Academic, New York, pp. 216–219.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Morna-Gomes, A., Serralheiro, M.L., Fevereiro, P.S. et al. Application of factorial design to the optimization of peroxidase activity in reverse micelles of bis(2-ethylhexyl)sodium sulfosuccinate/isooctane. Appl Biochem Biotechnol 82, 27–36 (1999). https://doi.org/10.1385/ABAB:82:1:27
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1385/ABAB:82:1:27