Abstract
Resting cells of Streptomyces clavuligerus NP-1, which posses deacetoxy-cephalosporin C synthase activity, have been shown previously to perform oxidative ring expansion of penicillin G in the presence of iron, ascorbic acid, and α-ketoglutaric acid to form deacetoxycephalosporin G. Further studies on this bioconversion indicated that use of MOPS or HEPES buffer at pH 6.5 more than doubled the extent of the reaction observed with the previously used Tris-HCl at pH 7.4. Levels of bioconversion as high as 16.5% were achieved at low penicillin G concentrations. Previously, conversion yields were <1%.
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References
Kohsaka, M. and Demain, A. L. (1976), Biochem. Biophys. Res. Commun. 70, 465–473.
Yoshida, M., Konomi, T., Kohsaka, M., Baldwin, J. E., Herchen, S., Singh, P., Hunt, N. A., and Demain, A. L. (1978), Proc. Natl. Acad. Sci. USA 75, 6253–6257.
Sawada, Y., Hunt, N. A., and Demain, A. L. (1979), J., Antibiot. 32, 1303–1310.
Felix, H. R., Peter, H. H., and Treichler, H. J. (1981), J. Antibiot. 34, 567–575.
Jensen, S. E., Westlake, D. W. S., Bowers, R. J., and Wolfe, S. (1982), J. Antibiot. 35, 1351–1360.
Kupka, J., Shen, Y., Wolfe, S., and Demain, A. L. (1983), Can. J. Microbiol. 29 488–496.
Baldwin, J. E. and Crabbe, M. J. C. (1987), FEBS Lett. 214, 357–361.
Maeda, K., Luengo, J. M., Ferrero, O., Wolfe, S., Lebedev, M. Y., Fang, A., and Demain, A. L. (1995), Enzyme Microb. Technol. 17, 231–234.
Dotzlaf, J. E. and Yeh, W. (1989), J. Biol. Chem. 264, 10,219–10,227.
Kupka, J., Shen, Y., Wolfe, S., and Demain, A. L. (1983), FEMS Microbiol. Lett. 16, 1–6.
Cho, H., Adrio, J. L., Luengo, J. M., Wolfe, S., Ocran, S., Hintermann, G. Piret, J. M., and Demain, A. L. (1998), Proc. Natl. Acad. Sci. USA 95, 11,544–11,548.
Mahro, B. and Demain, A. L. (1987), Appl. Microbiol. Biotechnol. 27, 272–275.
Shen, Y. Q., Wolfe, S. and Demain, A. L. (1984), Enzyme Microbiol. Technol. 6, 402–404.
Adrio, J. L., Cho, H., Piret, J. M. and Demain, A. L. (1999), Enzyme Microb. Technol. 25, 497–501.
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Báez-Vásquez, M.A., Adrio, J.L., Piret, J.M. et al. Further studies on the bioconversion of penicillin G into deacetoxycephalosporin G by resting cells of Streptomyces clavuligerus NP-1. Appl Biochem Biotechnol 81, 145–152 (1999). https://doi.org/10.1385/ABAB:81:3:145
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DOI: https://doi.org/10.1385/ABAB:81:3:145