Skip to main content
SpringerLink
Log in

Screening of variables in xylananese recovery using BDBAC reversed micelles

  • Published:
Applied Biochemistry and Biotechnology Aims and scope Submit manuscript

Abstract

Xylanase recovery by reversed micelles using cationic surfactants (N-benzyl-N-dodecyl-N-bis(2-hydroxyethhyl)ammonium chloride) BDBAC was evaluated under different experimental conditions. A full factorial design with center point was employed to verify the in fluence of different factors on the recovery. A mathematical model was found to represent the xylanese yield (Y) as a function of BDBAC and hexanol: Y=4.32+5.1B+2.64D+0.83B2+1.46D2, where B=BDBAC and D=hexanol. The highest xylanase recovery (27%), indicated by the model was attained at pH=8.1, BDBAC=0.38 M and hexanol=8.6%. Under these conditions, and to test the model, a new xylanase extraction was performed in laboratory, giving 29.4% recovery yield, this value was similar to that predicted by the model.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Duran, N., Milagres, A. M. F., Esposito, E., and Haun, M. (1995), in Enzymatic Degradation of Insoluble Carbohydrates, Saddler, J. N. and Ponner, M. H., eds., American Chemical Society, Washington, DC, pp. 332–338.

    Google Scholar 

  2. Milagres, A. M. F. and Prade, R. A. (1994), Enzyme Microbiol. Technol. 16, 627–631.

    Article  CAS  Google Scholar 

  3. Yang, J. L. and Eriksson, K. E. L. (1992), Holzforschung 46, 481–488.

    Article  CAS  Google Scholar 

  4. Eriksson, K. E. L. (1990) Wood Sci. Technol. 24, 79–101.

    Article  CAS  Google Scholar 

  5. Linko, M., Poutanen, K., and Viikari, L. (1989), in Enzyme Systems for Lignocellulose Degradation, Coughan, M. P., ed., Elsevier Applied Science, NY, pp. 331–346.

    Google Scholar 

  6. Vicuna, R., Oyarzun, E., and Osses, M. (1995), J. Biotechnol. 40(3), 163–168.

    Article  CAS  Google Scholar 

  7. Wong, K. K. Y., Nelson, S. L., and Saddler, J. N. (1996), J. Biotechnol. 48(1–2), 137–145.

    Article  CAS  Google Scholar 

  8. Senior, D. J., Hamilton, J., Bernier, R. L., and Dumanoir, J. R. (1992), Tappi J. 11, 125–130.

    Google Scholar 

  9. Tolan, J. S. and Canovas, R. V. (1992), Pulp Paper Can. 93, 39–40.

    CAS  Google Scholar 

  10. Viikari, L., Sundquist, J., and Kettunen, J. (1991), Paper Timber 73, 384–389.

    CAS  Google Scholar 

  11. Mutsaers, J. H. G. M. (1991), in Xylans and Xylanases International Symposium, Amsterdam, Elsevier, p. 48.

    Google Scholar 

  12. Biely, P. (1985), Trends Biotechnol. 3, 286–290.

    Article  CAS  Google Scholar 

  13. Wong, K. K. Y., Tan, L. U. L., and Saddler, J. N. (1988), Microbiol. Rev. 52(13), 305–317.

    PubMed  CAS  Google Scholar 

  14. Gattinger, L. D., Duvnjak, Z., and Khan, A. W. (1990), Appl. Microbiol. Biotechnol. 33, 21–25.

    Article  CAS  Google Scholar 

  15. Palma, M. B., Milagres, A. M. F., Prata, A. M. R., and Mancilha, J. M. (1996), Process Biochem. 141–145.

  16. Brandani, V., Di Giacomo, G., and Spera, L. Process Biochem. 31(2), 125–128.

  17. Krei, G. A. and Hustedt, H. (1992), Chem. Eng. Sci. 47, 99–111.

    Article  CAS  Google Scholar 

  18. Dekker, M., Van't Riet, K., and Weijers, S. R. (1986), Chem. Eng. J. 33, B27-B33.

    Article  Google Scholar 

  19. Pessoa, A., Jr. and Vitolo, M. (1997), Biotechnol. Tech. 11(6), 421–422.

    Article  CAS  Google Scholar 

  20. Leser, M. E. and Luisi, P. L. (1990), Chimia 44, 270–282.

    CAS  Google Scholar 

  21. Jolivalt, C., Minier, M., and Renon, H. (1990), J. Coll. Inter. Sci. 135, 85–96.

    Article  CAS  Google Scholar 

  22. Pessoa, A., Jr. (1995) Tese de Doutorado, USP, São Paulo.

    Google Scholar 

  23. Krei, G. A., Meyer, U., Borner, B., and Hustedt, H. (1995), Bioseparation 5, 175–183.

    CAS  Google Scholar 

  24. Krei, G. A. (1993), PhD Thesis, Technical University Braunschweig, Braunschweig, Germany.

  25. Brandani, V., Di Giacomo, G., and Spera, L. (1994), Process Biochem. 29, 363–367.

    Article  CAS  Google Scholar 

  26. Barros Neto, B., Scarminio, I. S., and Bruns, R. E. (1995), Planejamento e Otimização de Experimentos, Editora da Unicamp, Campinas.

    Google Scholar 

  27. Milagres, A. M. F. (1988), Tese de Mestrado UFV, Viçosa.

    Google Scholar 

  28. Vogel, H. J. (1956), Microbial Genet. Bull. 13, 42–43.

    Google Scholar 

  29. Milagres, A. M. F. and Lacis, L. S. (1991), Biotechnom. Lett. 13, 113–118.

    Article  CAS  Google Scholar 

  30. Bailey, M. J., Biely, P., and Poutanen, K. (1992), J. Biotechnol. 23(3), 257–270.

    Article  CAS  Google Scholar 

  31. Miller, G. L. (1959), Anal. Chem. 31, 426–428.

    Article  CAS  Google Scholar 

  32. Tsujibo, H., Sakamoto, T., Nishino, N., and Hasegawa, T. (1990), J. Appl. Bacteriol. 69, 398–405.

    CAS  Google Scholar 

  33. Honda, H., Kudo, T., Ikura, Y., and Horikoshi, K. (1985), Can. J. Microbiol. 31, 538–542.

    Article  CAS  Google Scholar 

  34. Gorbacheva, I. V. and Rodionova, N. A. (1977), Biochem. Biophys. Acta 484, 79–83.

    PubMed  CAS  Google Scholar 

  35. Subramani, S., Shah, C., and Madamwar, D. (1996), Appl. Biochem. Biotechnol. 60, 33–39.

    PubMed  CAS  Google Scholar 

  36. Blanch, H. W. (1990), Ferment. Technol. Ind. Appl. 329–336.

Download references

Author information

Authors and Affiliations

  1. Department of Biotechnology, Faculty of Chemical Engineering of Lorena, 12.600-000, Lorena, SP, Brazil

    E. M. G. Rodrigues, A. Pessoa Jr. & A. M. F. Milagres

Authors
  1. E. M. G. Rodrigues
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. Pessoa Jr.
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. A. M. F. Milagres
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to A. M. F. Milagres.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Rodrigues, E.M.G., Pessoa, A. & Milagres, A.M.F. Screening of variables in xylananese recovery using BDBAC reversed micelles. Appl Biochem Biotechnol 79, 779–788 (1999). https://doi.org/10.1385/ABAB:79:1-3:779

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1385/ABAB:79:1-3:779

Index Entries

Search

Navigation