Skip to main content
SpringerLink
Log in

Characterization and utilization of Candida rugosa lipase immobilized on controlled pore silica

  • Published:
Applied Biochemistry and Biotechnology Aims and scope Submit manuscript

Abstract

Candida rugosa lipase was immobilized by covalent binding on controlled poresilica (CPS) using glutaraldehyde ascross-linking agent under aqueous and nonaqueous conditions. The immobilized C. rugosa was more active when the coupling procedure was performed in the presence of a nonpolar solvent, hexane. Similar optima pH (7.5–8.0) was found for both free and immobilized lipase. The optimum temperature for the immobilized lipase was about 10°C higher than that for the free lipase. The thermal stability of the CPS lipase was alsogreater than the original lipase preparation. Studies on the operational stability of CPS lipase revealed good potential for recycling under aqueous (olive-oil hydrolysis) and nonaqueous (butyl butyrate synthesis) conditions.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Leuenberger, H. G. W. (1990), Pure Appl. Chem. 62, 753–768.

    CAS  Google Scholar 

  2. Zaks, A. and Klibanov, A. M. (1988), J. Biol. Chem. 263, 3194–3201.

    PubMed  CAS  Google Scholar 

  3. Castro, H. F. and Anderson, W. A. (1995), Qu'mica Nova. 18, 544–554.

    Google Scholar 

  4. Balcão, V. M., Paiva, A. L., and Xavier Malcata, F. (1996), Enzyme Microb. Technol 18, 392–416.

    Article  PubMed  Google Scholar 

  5. Faber, K. (1997). Biotransformation in Organic Chemistry: A Textbook, Springer-Verlag, Berlin.

    Google Scholar 

  6. Castro, H. F., Pereira, E. B., and Anderson, W. A. (1996), J. Brazilian Chem. Soc. 7, 219–224.

    Google Scholar 

  7. Castro, H. F., Oliveira, P. C., and Pereira, E. B. (1997), Biotechnol. Lett. 9, 229–232.

    Article  Google Scholar 

  8. Castro, H. F., Oliveira, P. C., and Soares, C. M. F. (1997), Bol. SBCTA. 17, 237–241.

    CAS  Google Scholar 

  9. Xavier Malcata, E., Reyes, H. R., Garcia, H. S., Hill, C. G., Jr., and Amundson, C. H. (1990), J. Am. Oil Chem. Soc. 67, 890–910.

    Article  Google Scholar 

  10. Mattiasson, B. and Adlecrentz, P. (1991), Tibtech. 9, 394–398.

    CAS  Google Scholar 

  11. Reslow, M., Adlercreutz, P., and Mattiason, B. (1988), European J. Biochem. 172, 573–578.

    Article  CAS  Google Scholar 

  12. Norin, M., Boutelje, J., Holmberg, E., and Hurt, K. (1988), Appl. Microbiol. Biotechnol. 28, 527–530.

    Article  CAS  Google Scholar 

  13. Wehtje, E., Adlercrentz, P., and Mattiason, B. (1993), Biotechnol. Bioeng. 41, 171–178.

    Article  CAS  Google Scholar 

  14. Ison, A. P., Macrae, A. R., Smith, C. G., and Basley, J. (1994), Biotechnol. Bioeng. 43, 122–130.

    Article  CAS  Google Scholar 

  15. Zanin, G. M. and De Moraes, F. F. (1994), Appl. Biotechnol. 45–46, 627–640.

    Google Scholar 

  16. Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J. (1951), J. Biol. Chem. 193, 263–275.

    Google Scholar 

  17. Baron, M., Florencio, J. A., Zanin, G. M., Ferreira, A. G., Ennes, R., and Fontana, J. D. (1996), Appl. Biotechnol. 57–58, 605–615.

    Article  Google Scholar 

  18. Mustranta, A., Forssell, P., and Poutanen, K. (1993), Enzyme Microb. Technol. 15, 133–139.

    Article  PubMed  CAS  Google Scholar 

  19. Gillies, B., Yamazaki, H., and Almstrong, D. (1987), Biotechnol. Lett. 9, 709–714.

    Article  CAS  Google Scholar 

  20. Carta, G., Gainer, J. L., and Benton, A. H. (1991), Biotechnol. Bioeng. 37, 1004–1009.

    Article  CAS  Google Scholar 

  21. Bosley, J. A. and Clayton, J. C. (1994), Biotechnol. Bioeng. 43, 934–938.

    Article  CAS  Google Scholar 

  22. Reetz, M. T., Zonta, A., and Simpel Kamp, J. (1996), Biotechnol. Bioeng. 49, 527–534.

    Article  CAS  Google Scholar 

  23. Laane, C., Boeren, S., Hilhorst, R., and Veeger, C. (1986), in Biocatalysis in Organic Media, Laane, C., Tramper, J., and Lilly, M. D., eds., Elsevier, Amsterdam, pp. 65–84.

    Google Scholar 

  24. Fukunaga, K., Minamijima, N., Sugimura, Y., Zhang, Z., and Nakao, K. (1996), J. Biotechnol. 52, 81–88.

    Article  CAS  Google Scholar 

  25. Castro, H. F., Oliveira, P. C., Soares, C. M. F., and Zanin, G. M. (1999), J. Am. Oil Chem. Soc. 76 (1), in press.

Download references

Author information

Authors and Affiliations

  1. Department of Chemical Engineering, Faculty of Chemical Engineering of Lorena, PO Box 116, 12600-000, Lorena, SP, Brazil

    Cleide M. F. Soares & Heizir F. De Castro

  2. Department of Chemical Engineering, Maringá State University, Maringá-PR, Brazil

    Flávio F. De Moraes & Gisella M. Zanin

Authors
  1. Cleide M. F. Soares
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Heizir F. De Castro
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Flávio F. De Moraes
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Gisella M. Zanin
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Heizir F. De Castro.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Soares, C.M.F., De Castro, H.F., De Moraes, F.F. et al. Characterization and utilization of Candida rugosa lipase immobilized on controlled pore silica. Appl Biochem Biotechnol 79, 745–757 (1999). https://doi.org/10.1385/ABAB:79:1-3:745

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1385/ABAB:79:1-3:745

Index Entries

Search

Navigation