Applied Biochemistry and Biotechnology

, Volume 79, Issue 1–3, pp 689–699 | Cite as

Expression of Trichoderma reesei exo-cellobiohydrolase I in transgenic tobacco leaves and calli

  • Ziyu Dai
  • Brian S. Hooker
  • Ryan D. Quesenberry
  • Jianwei Gao


Expression of Trichoderma reesei exo-cellobiohydrolase I (CBHI) gene in transgenic tobacco was under the control of CaMV 35S promoter. In transgenic leaf tissues, CBHI activity up to 66.1 μmol/h/g total protein was observed. In transgenic calli, the highest CBHI activity was 83.6 μmol h/g total protein. Protein immunoblot analysis confirms the presence of CBHI enzyme in both transgenic calli and leaf tissues. CBHI expression levels accounted for about 0.11% and 0.082% of total protein in transgenic leaf tissues and calli, respectively, Furthermore, expression of CBHI gene did not affect normal growth and development of transgenic plants.

Index Entries

Bioreactor cellulase exo-cellobiohydrolase I transgenic tobacco Trichoderma reesei 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    An, G., Ebert, P. R., and Ha, S.-B. (1988), in Plant Molecular Biology Manual, Gelvin, S. B. and Schilperoort, R. A., eds., Kluwer, Dordrecht, The Netherlands, pp. 1–19.Google Scholar
  2. 2.
    An, G. (1995), in Methods in Molecular Biology, vol 44, Gartland, K. M. A. and Davey, M. R., eds. Humana Press, Totowa, NJ, pp. 47–58.Google Scholar
  3. 3.
    Austin, S., Bingham, E. T., Koegel, R. G., Mathews, D. E., Shahan, M. N., Straub, R. J., and Burgess, R. R. (1994), Ann. NY Acad. Sci. 721, 234–244.PubMedCrossRefGoogle Scholar
  4. 4.
    Dai, Z., Ku, M. S. B., Zhang, D., and Edwards, G. E. (1994), Planta 192, 287–294.CrossRefGoogle Scholar
  5. 5.
    Duff, S. J. and Murray, W. D. (1996), Biores. Technol. 55, 1–33.CrossRefGoogle Scholar
  6. 6.
    Herbers, K., Wilke, I., and Sonnewald, U. (1995), Bio/Technology 13, 63–66.CrossRefGoogle Scholar
  7. 7.
    Hoekema, A., Hirsch, P. R., Hooykaas, P. J. J., and Schilperoort, R. A. (1983), Nature 303, 179–181.CrossRefADSGoogle Scholar
  8. 8.
    Hood, E. E., Witcher, D. R., Maddock, S., Meyer, T., Baszczynski, C., Bailey, M., Flynn, P., Register, J., Marshall, L., Bond, D., Kullisek, E., Kusnadi, A., Evangelista, R., Nikolov, Z., Wooge, C., Mehigh, R. J., Herman, R., Kappel, W. K., Ritland, D., Li, C. P., and Howard, J. A. (1997), Mol. Breeding 3, 291–306.CrossRefGoogle Scholar
  9. 9.
    Irwin, D. C., Spezio, M., Walker, L. P., and Wilson, D. B. (1993), Biotechnol. Bioengin. 42, 1002–1013.CrossRefGoogle Scholar
  10. 10.
    Murashige, T. and Skoog, F. (1962), Physiol. Plant 15, 473–497.CrossRefGoogle Scholar
  11. 11.
    Pen, J., Sijmons, P. C., van Ooijen, A. J. J., and Hoekema, A. (1993), in Industrial Crops Production, Elsevier, Amsterdam, pp. 241–250.Google Scholar
  12. 12.
    Sambrook, J., Fritsch, E. F., and Maniatis, T. (1989), Molecular Cloning: A Laboratory Manual, 2nd ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.Google Scholar
  13. 13.
    Shoemaker, S., Watt, K., Tsitovsky, G., and Cox, R. (1983a), Biotechnology 1, 687–690.CrossRefGoogle Scholar
  14. 14.
    Shoemaker, S., Schweickart, V., Landner, M., Gelfand, D., Kwok, S., Myambo, K., and Innis, M. (1983b), Biotechnology 1, 691–696.CrossRefGoogle Scholar
  15. 15.
    Stewart, C. N., Jr. and Via, L. E. (1993), Biotechniques 14, 748–750.PubMedGoogle Scholar
  16. 16.
    Terri, T., Salovuori, I., and Knowles, J. (1983), Biotechnology 1, 697–702.Google Scholar
  17. 17.
    Van Etten, J. L., Freer, S. N., and McCune, (1987), J. Bacteriol. 138, 650–652.Google Scholar
  18. 18.
    Verwoerd, T. C., van Paridon, P. A., Van Ooyen, A. J. J., vanLent, J. W. M., Hoekema, A., and Pen, J. (1994), Plant Physiol. 109, 1199–1203.CrossRefGoogle Scholar
  19. 19.
    Whitelam, G. C., Cockburn, B., Gandeacha, A. R., and Owen, M. R. L. (1993), Biotechnol. Genet. Eng. Rev. 11, 1–29.PubMedGoogle Scholar

Copyright information

© Humana Press Inc. 1999

Authors and Affiliations

  • Ziyu Dai
    • 1
  • Brian S. Hooker
    • 1
  • Ryan D. Quesenberry
    • 1
  • Jianwei Gao
    • 1
  1. 1.Bioprocessing Group, Environmental Technological DivisionPacific Northwest National LaboratoryRichland

Personalised recommendations