Applied Biochemistry and Biotechnology

, Volume 78, Issue 1–3, pp 571–575 | Cite as

A preliminary information about continuous fermentation using cell recycling for improving microbial xylitol production rates

Scientific note
  • Silvio S. SilvaEmail author
  • Adolfo Q. Chanto
  • Michele Vitolo
  • Maria G. A. Felipe
  • Ismael M. Mancilha


Xylitolis a sugar-alcohol with important technological properties, such as anticariogenicity, low caloric value, and negative dissolution heat. It can be used successfully in food for mulations and pharmaceutical industries. Its production is therefore in great demand. Biotechnological xylitol production has several economic advantages in comparison with the conventional process based on the chemical reduction of xylose. The efficiency and the productivity of this fermentation chiefly depends on the microorganism and the process conditions employed. In this article a simple continuous culture with cell recycling was evaluated to enhance the capability of Candida guilliermondii FTI 20037 to produce xylitol. The fermentation was initiated batchwise by directly inoculating the grown seed culturein a 2-L bench-scale fermentor. Continuous feeding was begun at a dilution rate (D) of 0.060/h after the xylose concentration had completely consumed and the cell concentration was a bout 4.0 g/L. At a dilution rate of 0.060/h the xylitol concentration was about 15g/L and in creased by about 35%, whereas the dilution rate decreased by about 58%. Furthermore, the volumetric productivity, Qp, markedly depended on the dilution rate, diminishing by about 37% as D was changed from 0.060 to 0.025/h. These preliminary results show us that the continous fermentation with cell recycling is a good way to study the xylitol production by xylose-fermenting yeasts.

Index Entries

Xylitol yeast continous fermentation recycling 


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Copyright information

© Humana Press Inc. 1999

Authors and Affiliations

  • Silvio S. Silva
    • 1
    Email author
  • Adolfo Q. Chanto
    • 2
  • Michele Vitolo
    • 3
  • Maria G. A. Felipe
    • 1
  • Ismael M. Mancilha
    • 1
  1. 1.Department of Biotechnology, Faculty of Chemical Engineering of LorenaFAENQUIL/DEBIQLorena-São PauloBrazil
  2. 2.Biotechnological Development Center (CDB)JoinvilleBrazil
  3. 3.Department of Biochemical and Pharmaceutical TechnologyUniversity of São PauloBrazil

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