Applied Biochemistry and Biotechnology

, Volume 78, Issue 1–3, pp 455–472 | Cite as

Bioconversion of mixed solids waste to ethanol

  • Quang A. NguyenEmail author
  • Fred A. Keller
  • Melvin P. Tucker
  • Charles K. Lombard
  • Bryan M. Jenkins
  • David E. Yomogida
  • Valentino M. Tiangco


A mixed solids waste (MSW) feedstock, comprising construction lumber waste (35% oven-dry basis), alm ond treeprunings (20%), wheat straw (20%), office waste paper (12.5%), and newsprint (12.5%), was converted to ethanol via dilute-acid pretreatment followed by enzymatic hydrolysis and yeast fermentation. The MSW was pretreated with dilute sulfuricacid (0.4% w/w) at 210°C for 3 min in a 4-L stea mexplosion reactor, then washed with water to recover the solubilized hemicellulose. The digestibility of water-washed, pretreated MSW was 90% in batch enzymatic hydrolysis at 66 FPU/g cellulose. Using an enzyme-recycle bioreactor system, greater than 90% cellulose hydrolysis was achieved at a net enzyme loading of about 10 FPU/g cellulose. Enzyme recycling using mebrane filtration and a fed-batch fermentation technique is a promising option for significantly reducing the cost of enzyme in cellulose hydrolysis. The hexosesugars were readily fermentable using a Saccharomyces cerevisiae yeast strain that was adapted to the hydrolysate. Solid residue after enzyme digestion was subjected to various furnace experiments designed to assess the fouling and slagging characteristics. Results of these analyses suggest the residue to be of a low to moderate slagging and fouling type if burned by itself.

Index Entries

Biomass ethanol pretreament MSW bioconversion hydrolysis enzyme recycle 


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Copyright information

© Humana Press Inc. 1999

Authors and Affiliations

  • Quang A. Nguyen
    • 1
    Email author
  • Fred A. Keller
    • 1
  • Melvin P. Tucker
    • 1
  • Charles K. Lombard
    • 2
  • Bryan M. Jenkins
    • 3
  • David E. Yomogida
    • 3
  • Valentino M. Tiangco
    • 4
  1. 1.National Renewable Energy LaboratoryGolden
  2. 2.Waste Energy Integrated SystemsPalo Alto
  3. 3.Biological and Agricultural EngineeringUniversity of CaliforniaDavis
  4. 4.California Energy CommissionSacramento

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