Applied Biochemistry and Biotechnology

, Volume 78, Issue 1–3, pp 445–454 | Cite as

Conversion of industrial food wastes by Alcaligenes latus into polyhydroxyalkanoates

  • Peter H. YuEmail author
  • Hong Chua
  • Ai-Ling Huang
  • Kwok-Ping Ho


Broader usage of biodegradable plastics in packaging and disposable products as a solution to environmental problems would heavily depend on further reduction of costs and the discovery of novel biodegradable plastics with improved properties. As the first step in our pursuit of eventual usage of industrial food wastewater as nutrients for microorganisms to synthesise environmental-friendly bioplastics, we investigated the usage of soya wastes from a soya milk dairy, and malt wastes from a beer brewery plant as the carbon sources for the production of polyhydroxyalkanoates (PHA) by selected strain of microorganism. Bench experiments showed that Alcaligenes latus DSM 1124 used the nutrients from malt and soya wastes to biosynthesise PHAs. The final dried cell mass and specific polymer production of A. latus DSM 1124 were 32g/L and 70% polymer/cells (g/g), 18.42 g/L and 32.57% polymer/cell (g/g), and 28 g/L and 36% polymer/cells (g/g), from malt waste, soya waste, and from sucrose, responctively. These results suggest that many types of food wastes might be used as the carbon source for the production of PHA.

Index Entries

Polyhydroxyalkanoate (PHA) polyhydroxybutyrate (PHB) malt soya waste Alcaligenes Alcaligenes latus 


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  1. 1.
    Byrom, D. (1994), in Plastics from microbes: microbial synthesis of polymers and polymer precursors, Mobley, D. P., ed., Hanser, Munich, pp. 5–11.Google Scholar
  2. 2.
    Yu, P. and Giuliany, B. (1989), Frontiers 9 (11), 4–7.Google Scholar
  3. 3.
    Lee, S. Y. (1995), Biotechnol. Bioeng. 49, 1–14.CrossRefGoogle Scholar
  4. 4.
    Helmut, B., Gross, R. A., Lenz, R. W., and Fuller, R. C. (1990), Adv. Biochem. Eng./Biotechnol. 41, 78–91.Google Scholar
  5. 5.
    Linko, S., Vaheri, H., and Seppala, J. (1993), Enzyme Microbiol. Technol. 15(5), 401–406.CrossRefGoogle Scholar
  6. 6.
    Lee, S. Y. (1996), Trends Biotechnol. 14, 431–438.CrossRefGoogle Scholar
  7. 7.
    Yamane, T., Fukunagga, M., and Lee, Y. W. (1996), Biotechnol. Bioeng. 50(2), 197–202.CrossRefGoogle Scholar
  8. 8.
    Kim B. S. and Chang, H. S. (1995), Biotechnol. Techniques 9(5), 311–314.CrossRefGoogle Scholar
  9. 9.
    Alderte, J. E., Karl, D. W., and Park, C. H. (1993), Biotechnol. Prog. 9(5), 520–525.CrossRefGoogle Scholar
  10. 10.
    Shah N. N., Hanna, M. L., and Taylor, R. T. (1996), Biotechnol. Bioeng. 49(2), 161–171.CrossRefGoogle Scholar
  11. 11.
    Son, H. and Lee, S. (1996), Biotechnology Lett. 18, 1217–1222.CrossRefGoogle Scholar
  12. 12.
    Yu, P. H., Chua, H., and Ho, L. Y. (1996), Proceedings of the Asia-Pacific Conference on Sustainable Energy and Environ mental Technology, Singapore, pp. 623–630.Google Scholar
  13. 13.
    Yu, P. H., Chua, H., Huang, A. L., Lo, W., and Chen, J. C. (1998), Conversion of food industrial wastes into bioplastics. Appl. Biochem. Biotechnol.Google Scholar
  14. 14.
    Gomez, J. G. C., Rodrigues, M. F. A., Alli, R. C. P., Torres, B. B., Bueno Netto, C. L., Oliverira, M. S., and da Silva, L. F. (1996), Appl. Microbiol. Biotechnol. 45, 785–791.CrossRefGoogle Scholar
  15. 15.
    Hahn S. K., Chang, Y. K., Kim, B. S., and Chang, H. N. (1994), Biotechnol. Bioeng. 44(2), 256–261.CrossRefGoogle Scholar
  16. 16.
    Roh, K. S., Yeom, S. H., and Yoo, Y. J. (1995), Biotechnol. Techniques. 9(10), 709–771.CrossRefGoogle Scholar
  17. 17.
    Greenberg, A. E., Clesceri, L. S., and Eton, A. D. (1992), TKN analysis (5310C). Standard Methods for the Examination of Water and Wastewater, 18th ed., Apha, Washington, DC.Google Scholar
  18. 18.
    Jan, S., Roblot, C., Goethals, G., Courtois, J., Courtois, B., Saucedo, J. E. N., Seguin, J. P., and Barbotin, J. N. (1995), Anal. Biochem. 225, 258–263.CrossRefGoogle Scholar
  19. 19.
    Jan S., Roblot, C., Courtois, J., Courtois, B., Barbotin, J. N., and Seguin, J. P. (1996), Enzyme Microbiol. Technol. 18, 195–201.CrossRefGoogle Scholar
  20. 20.
    Hu, W. F., Chua, H., and Yu, P. H. F. (1997), Biotechnol. Lett. 19, 695–698.CrossRefGoogle Scholar
  21. 21.
    Chua, H., Yu, P. H. F., and Ho, L. Y. (1997), Appl. Biochem. Biotechnol. 63, 627–635.Google Scholar

Copyright information

© Humana Press Inc. 1999

Authors and Affiliations

  • Peter H. Yu
    • 1
    Email author
  • Hong Chua
    • 2
  • Ai-Ling Huang
    • 1
  • Kwok-Ping Ho
    • 1
  1. 1.Union Laboratory of Asymmetric Synthesis and Department of Applied Biology and Chemical TechnologyHong Kong Polytechnic UniversityHong Kong, China
  2. 2.Department of Civil and Structural EngineeringHong Kong Polytechnic UniversityHong Kong, China

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