Applied Biochemistry and Biotechnology

, Volume 77, Issue 1–3, pp 67–81 | Cite as

Modeling the enzymatic hydrolysis of dilute-acid pretreated Douglas Fir

  • Daniel J. SchellEmail author
  • Mark F. Ruth
  • Melvin P. Tucker


Glucose yield from the enzymatic hydrolysis of cellulose was investigated as a function of cellulase enzyme loading (7–36 filter paper units [FPU]/g cellulose) and solids concentration (7–18% total solids) for up to 72 h on dilute sulfuric-acid pretreated Douglas Fir. The saccharification was performed on whole hydrolysate with no separation or washing of the solids. Enzyme loading had a significant effect on glucose yield; solids concentration had a much smaller effect even at higher glucose concentrations. The data were used to generate an empirical model for glucose yield, and to fit parameters of a cellulose hydrolysis kinetic model. Both models could be used for economic evaluation of a separate hydrolysis and fermentation process.

Index Entries

Cellulose hydrolysis softwood ethanol enzyme kinetics cellulase 


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Copyright information

© Humana Press Inc. 1999

Authors and Affiliations

  • Daniel J. Schell
    • 1
    Email author
  • Mark F. Ruth
    • 1
  • Melvin P. Tucker
    • 1
  1. 1.National Renewable Energy LaboratoryGolden

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