Applied Biochemistry and Biotechnology

, Volume 77, Issue 1–3, pp 347–354 | Cite as

Aspects of xylitol formation in sugarcane bagasse hydrolysate by Candida guillie rmondii in the presence of tetracycline

  • Ernesto Acosta Martínez
  • Silvio S. SilvaEmail author
  • Maria G. A. Felipe


The biocon version of xylose intoxylitol using pH values of 4.0, 5.5 and 7.0 and tetracycline concentrations of 20 and 40 mg/L was carried out to verify the influence of these parameters on Candida guilliermondii metabolism for xylitol production. Experiments were performed with sugarcane bagasse hemicellulosi chydrolysate (48.5 g/L of xylose) in 125-mL Erlenmeyer flasks, at 30°C, 200 rpm, during 88 h. The results demostrated that the bioconversion of xylose into xylitol was significantly influenced by the pH. On the other hand, in media containing 20 or 40 mg/L of tetracycline, this bioconversion was not significantly affected. The best results of xylitol production were obtained in hemicellulosic hydrolysate without tetracycline, at pH 7.0 In these conditions, the maxim um specific growth rate was 0.014/h and the yield factor of xylitol and volumetric productivity were 0.85g/g and 0.70g/L/h respectively. Xylitol and cell growth occureed simultaneously.

Index Entries

Hemicellulosic hydrolysate sugarcane bagasse xylose xylitol Candida guilliermondii Klebsiella pueumoniae 


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Copyright information

© Humana Press Inc. 1999

Authors and Affiliations

  • Ernesto Acosta Martínez
    • 1
    • 2
  • Silvio S. Silva
    • 1
    Email author
  • Maria G. A. Felipe
  1. 1.Department of BiotechnologyFaculty of Chemical Engineering of LorenaLorenaBrasil
  2. 2.Cuban Institute for Research on Sugarcane DerivativesHavana CityCuba

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