Aspects of xylitol formation in sugarcane bagasse hydrolysate by Candida guillie rmondii in the presence of tetracycline
- 24 Downloads
The biocon version of xylose intoxylitol using pH values of 4.0, 5.5 and 7.0 and tetracycline concentrations of 20 and 40 mg/L was carried out to verify the influence of these parameters on Candida guilliermondii metabolism for xylitol production. Experiments were performed with sugarcane bagasse hemicellulosi chydrolysate (48.5 g/L of xylose) in 125-mL Erlenmeyer flasks, at 30°C, 200 rpm, during 88 h. The results demostrated that the bioconversion of xylose into xylitol was significantly influenced by the pH. On the other hand, in media containing 20 or 40 mg/L of tetracycline, this bioconversion was not significantly affected. The best results of xylitol production were obtained in hemicellulosic hydrolysate without tetracycline, at pH 7.0 In these conditions, the maxim um specific growth rate was 0.014/h and the yield factor of xylitol and volumetric productivity were 0.85g/g and 0.70g/L/h respectively. Xylitol and cell growth occureed simultaneously.
Index EntriesHemicellulosic hydrolysate sugarcane bagasse xylose xylitol Candida guilliermondii Klebsiella pueumoniae
Unable to display preview. Download preview PDF.
- 2.Silva, S. S. and Afschar, A. S. (1994), Bioprocess Eng. 11, 129–134.Google Scholar
- 7.Emodi, A. (1978), Food Technol. 1, 28–32.Google Scholar
- 13.Brock, T. D., T-Madigan, M., Martinko, J. M., and Parker, J. (1994), Biol. Microorg., 7th ed. Prentice-Hall International, UK, p. 909Google Scholar
- 14.Barros Neto, B., Scarminio, I. S., and Bruns, R. E. (1995), Planejamento e Otimização de experimentos, Campinas: editora da UNICAMP, Campinas. p. 299.Google Scholar
- 15.Mevrial, V., Delgenes, J. P., Molletta, R., and Navarro, J. M. (1991), Biotechnol. Lett. 13(1/2), 11–14.Google Scholar
- 16.Felipe, M. G. A., Vitolo, M., Mancilha, I. M., and Silva, S. S. (1997), Biotechnol. Lett. 13(4), 281–286.Google Scholar