Abstract
An expression vector for purple bacteria based on the puf promoter from Rhodobacter sphaeroides was constructed. The 1.2 kb promoter fragment included the pufQ gene, ORFK, as wellas the SD sequence and thestartcodon from pufB. Translational pufduc fusion was constructed toanalyze transcription from the puf promoter. The luciferase expression was negligible under aerobic light conditions and was stimulated more than 700-fold under anaerobic light conditions. When Rb. sphaeroides, (p Pluc-2) strain was grown photoheterotrophically in batch culture, the luciferase expression was observed during 7h. The expression time could be greatly extended using continuous cultivation in a photobioreactor.
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Vasilyeva, L., Miyake, M., Nakamura, C. et al. Expression of luciferase gene under control of the puf promoter from Rhodobacter sphaeroides . Appl Biochem Biotechnol 77, 337–345 (1999). https://doi.org/10.1385/ABAB:77:1-3:337
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DOI: https://doi.org/10.1385/ABAB:77:1-3:337