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Applied Biochemistry and Biotechnology

, Volume 77, Issue 1–3, pp 337–345 | Cite as

Expression of luciferase gene under control of the puf promoter from Rhodobacter sphaeroides

  • Lyudmila Vasilyeva
  • Masato Miyake
  • Chikashi Nakamura
  • Eiji Nakada
  • Anatoly Tsygankov
  • Yasuo AsadaEmail author
  • Jun Miyake
Article

Abstract

An expression vector for purple bacteria based on the puf promoter from Rhodobacter sphaeroides was constructed. The 1.2 kb promoter fragment included the pufQ gene, ORFK, as wellas the SD sequence and thestartcodon from pufB. Translational pufduc fusion was constructed toanalyze transcription from the puf promoter. The luciferase expression was negligible under aerobic light conditions and was stimulated more than 700-fold under anaerobic light conditions. When Rb. sphaeroides, (p Pluc-2) strain was grown photoheterotrophically in batch culture, the luciferase expression was observed during 7h. The expression time could be greatly extended using continuous cultivation in a photobioreactor.

Index Entries

Rhodobacter sphacroides expression vector puf promoter luciferase continuous culture 

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Copyright information

© Humana Press Inc. 1999

Authors and Affiliations

  • Lyudmila Vasilyeva
    • 1
    • 2
    • 3
    • 5
  • Masato Miyake
    • 1
  • Chikashi Nakamura
    • 4
  • Eiji Nakada
    • 3
  • Anatoly Tsygankov
    • 5
  • Yasuo Asada
    • 1
    Email author
  • Jun Miyake
    • 1
    • 4
  1. 1.National Institute of Bioscien ce and Human TechnologyTsukuba, IbarakiJapan
  2. 2.Research Institute of Innovative Technology for the EarthJapan
  3. 3.Fuji Electric Corporate R&D, Ltd.KanagawaJapan
  4. 4.National Institute of Advanced Interdisciplin ary ResearchIbarakiJapan
  5. 5.Institute of Basic Biological ProblemsRussian Academy of SciencePushchinoRussia

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