Construction and characterization of fermentative lactate dehydrogenase Escherichia coli mutant and its potential for bacterial hydrogen production
- 107 Downloads
In Escherichia coli, classified as a mixed-acid producer in fermentation, D-lactate is one of the final metabolites from pyruvate. In order to achieve a high efficiency of bacterial hydrogen production from glucose, we have constructed an E. coli strain deficient in fermentative lactate dehydrogenase (LDH-A) by P1 transduction. The mutant, designated as MC13-4, entirely lost LDH-A activity while retaining whole formate hydrogenlyase activity. This mutation resulted in an increase in hydrogen production based on glucose consumed. The effect of uptake hydrogenases on the hydrogen production was also discussed.
Index EntriesMetabolic engineering lactate dehydrogenase hydrogen production Escherichia coli
Unable to display preview. Download preview PDF.
- 6.Sawers, B. A. A. G. (1996), in Escherichia coli and Salmonella, 2nd ed., Neidhardt, F. C., et al. eds., ASM Press, Washington, DC, pp. 262–282.Google Scholar
- 8.Miller, J. H. (1992), in A Short Course in Bacterial Genetics, Miller, J. H., ed., pp. 263–274.Google Scholar
- 9.Tarmy, E. M. and Kapran, K. O. (1968), J. Biol. Chem. 10, 2579–2586.Google Scholar
- 10.Mat-Jan, F. A., Alam, K. Y., and Clark, D. P. (1989), J. Bacteriol. 171, 342–348.Google Scholar