Applied Biochemistry and Biotechnology

, Volume 77, Issue 1–3, pp 317–323 | Cite as

Construction and characterization of fermentative lactate dehydrogenase Escherichia coli mutant and its potential for bacterial hydrogen production

  • Koji SodeEmail author
  • Mika Watanabe
  • Hiroshi Makimoto
  • Masamitsu Tomiyama


In Escherichia coli, classified as a mixed-acid producer in fermentation, D-lactate is one of the final metabolites from pyruvate. In order to achieve a high efficiency of bacterial hydrogen production from glucose, we have constructed an E. coli strain deficient in fermentative lactate dehydrogenase (LDH-A) by P1 transduction. The mutant, designated as MC13-4, entirely lost LDH-A activity while retaining whole formate hydrogenlyase activity. This mutation resulted in an increase in hydrogen production based on glucose consumed. The effect of uptake hydrogenases on the hydrogen production was also discussed.

Index Entries

Metabolic engineering lactate dehydrogenase hydrogen production Escherichia coli 


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Copyright information

© Humana Press Inc. 1999

Authors and Affiliations

  • Koji Sode
    • 1
    Email author
  • Mika Watanabe
    • 1
  • Hiroshi Makimoto
    • 1
  • Masamitsu Tomiyama
    • 2
  1. 1.Department of BiotechnologyTokyo University of Agriculture and TechnologyTokyoJapan
  2. 2.National Institute of Agrobiological ResourcesTsukuba-city, IbarakiJapan

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