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Applied Biochemistry and Biotechnology

, Volume 77, Issue 1–3, pp 267–275 | Cite as

A high-copy-number plasmid capable of replication in thermophilic cyanobacteria

  • Masato Miyake
  • Hiroshi Nagai
  • Makoto Shirai
  • Ryuichiro Kurane
  • Yasuo AsadaEmail author
Article

Abstract

A 2.5 kb high-copy-number plasmid, pM A4 in thermophilic cyanobacterium Synechococcus sp. M A4 was isolated and characterized to develop a genetic engineering system for thermophilic cyanobacteria. The copy number of pM A4 was determined to be by densitometry about 350/cell. The pM A4 may be a type of rolling-circle plasmid, because a possible rep gene encoding 34 k D-protein and a consensus sequence of a double-stranded origin nick site of rolling circle plasmids were found in the pM A4 sequence. The pM A4 was electro-introduced into another thermophile, Synechococcus sp. MA 19, which is the strongest poly-β-hydroxybutyrate (PHB) accumulator in photoau totrophic organisms. The pM A4 was incorporated and retained in MA 19. These results indicate that pM A4 could be developed as a useful vector for thermophilic cyanobacteria.

Index Entries

Thermophilic cyanobacteria poly-β-hydroxybutyrate carbon dioxide fixation biodegradable plastic genetic engineering rolling circle plasmid 

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Copyright information

© Humana Press Inc. 1999

Authors and Affiliations

  • Masato Miyake
    • 1
  • Hiroshi Nagai
    • 2
  • Makoto Shirai
    • 2
  • Ryuichiro Kurane
    • 1
  • Yasuo Asada
    • 1
    Email author
  1. 1.National Institute of Bioscience and Human-Technology, Agency of Industrial Science and TechnologyMinistry of International Trade and IndustryTsukuba, IbarakiJapan
  2. 2.Division of BiotechnologyIbaraki UniversityIbarakiJapan

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