Abstract
A 2.5 kb high-copy-number plasmid, pM A4 in thermophilic cyanobacterium Synechococcus sp. M A4 was isolated and characterized to develop a genetic engineering system for thermophilic cyanobacteria. The copy number of pM A4 was determined to be by densitometry about 350/cell. The pM A4 may be a type of rolling-circle plasmid, because a possible rep gene encoding 34 k D-protein and a consensus sequence of a double-stranded origin nick site of rolling circle plasmids were found in the pM A4 sequence. The pM A4 was electro-introduced into another thermophile, Synechococcus sp. MA 19, which is the strongest poly-β-hydroxybutyrate (PHB) accumulator in photoau totrophic organisms. The pM A4 was incorporated and retained in MA 19. These results indicate that pM A4 could be developed as a useful vector for thermophilic cyanobacteria.
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Miyake, M., Nagai, H., Shirai, M. et al. A high-copy-number plasmid capable of replication in thermophilic cyanobacteria. Appl Biochem Biotechnol 77, 267–275 (1999). https://doi.org/10.1385/ABAB:77:1-3:267
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DOI: https://doi.org/10.1385/ABAB:77:1-3:267