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Applied Biochemistry and Biotechnology

, Volume 77, Issue 1–3, pp 191–204 | Cite as

Fermentation performance characteristics of a prehydrolyzate-adapted xylose-fermenting recombinant Zymomonas in batch and continuous fermentations

  • Hugh G. LawfordEmail author
  • Joyce D. Rousseau
  • Ali Mohagheghi
  • James D. McMillan
Article

Abstract

Long-term (149 d) continuous fermentation was used to adapt a xylose-fermenting recombinant Zymomonas mobilis, strain 39676:pZB 4L, to conditioned (overlimed) dilute-acid yellow poplar hemicellulose hydrolyzate (“prehydrolyzate”). An “adapted” variant was isolated from a chemostat operating at a dilution rate of 0.03/h with a 50% (v/v) prehydrolyzate, corn steep liquor, and sugar-supplemented medium, at pH 5.75. The level of xylose and glucose in the medium was kept constant at 4% (w/v) and 0.8% (w/v), respectively. These sugar concentrations reflect the composition of the undiluted hardwood prehydrolyzate. The level of conditioned hardwood prehydrolyzate added to the medium was increased in 5% increments startingata level of 10%. At the upper level of 50% prehydrolyzate, the acetic-acid concentration was about 0.75% (w/v). The adapted variant exhibited improved xylose-fermentation performance in a pure-sugar, synthetic hardwood prehydrolyzate medium containing 4% xylose (w/v), 0.8% (w/v) glucose, and acetic acid in the range 0.4–1.0% (w/v). The ethanol yield was 0.48–0.50 g/g; equivalent to a sugar-to-ethanol conversion efficiency of 94–96% of theoretical maximum. The maximum growth yield and maintenance energy coefficients were 0.033 g dry cell mass (DCM)/g sugars and 0.41 g sugars/g DCM/h, respectively. The results confirm that long-term continuous adaptation is a useful technique for effecting strain improvement with respect to the fermentation of recalcitrant feedstocks.

Index Entries

Recombinant Zymomonas continuous cofermentation xylose hardwood prehydrolyzate ethanol yield adaptation acetic acid 

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Copyright information

© Humana Press Inc. 1999

Authors and Affiliations

  • Hugh G. Lawford
    • 1
    Email author
  • Joyce D. Rousseau
    • 1
  • Ali Mohagheghi
    • 2
  • James D. McMillan
    • 2
  1. 1.Bioengineering Laboratory, Department of BiochemistryUniversity of TorontoTorontoCanada
  2. 2.Biotechnology Center for Fuels and ChemicalsNational Renewable Energy LaboratoryGolden

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