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Applied Biochemistry and Biotechnology

, Volume 77, Issue 1–3, pp 169–179 | Cite as

Isolation and analysis of tetraheme-bound-cytochrome from photosynthetic reaction centers of Rhodopseudomonas viridis

  • Chikashi NakamuraEmail author
  • Miki Hasegawa
  • Masayuki Hara
  • Jun Miyake
Article
  • 26 Downloads

Abstract

A tetraheme cytochrome (BCytc) was isolated from the photosynthetic reaction centers (RC) of Rhodopseudomonas viridis while maintaining the redox activity. BCytc was removed from the H-subunit-detached RC by polyacrylamide electrophoresis using an alkyl ether sulfate mixed with sodium dodecyl sulfate. Redox titration of BCytc showed a simple one-step redox titration curve and a lowered midpoint potential than that of one in RC. Direct electron transfer between BCytc and electrode surfaces, such as indium tin oxide, was successfully performed, indicating a potential for molecular electronic material.

Index Entries

Tetraheme cytochrome surfactant photosynthetic reaction center photosynthetic bacterium 

Abbreviations

BCytc

the bound cytochrome c

RC

photosynthetic reaction centers

RC−H

H-subunit-detached RC

AES

sodium polyoxyethylene alkyl ether sulfate

SDS

Sodium dodecyl sulfate

β-OG

n-octyl-β-d-glucoside

HNQ

2-hydroxy-1,4-naphthoquinone

TNTB

tetranitro tetrazolium blue

DAD

diaminodurene

PMS

phenazine methosulfate

ITO

indium tin oxide

SHE

standard hydrogen electrode

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Copyright information

© Humana Press Inc. 1999

Authors and Affiliations

  • Chikashi Nakamura
    • 1
    Email author
  • Miki Hasegawa
    • 1
  • Masayuki Hara
    • 1
  • Jun Miyake
    • 1
  1. 1.National Institute for Advanced Interdisciplinary Research, Agency of Industrial Science and TechnologyMinistry of International Trade and IndustryHigashi, TsukubaJapan

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