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Improved activity in acidic media of immobilized lysozyme

  • Marina Zacchigna
  • Gabriella Di Luca
  • Lucia Lassiani
  • Antonio Varnavas
  • Anna Pitotti
  • Enrico BoccùEmail author
Article

Abstract

Polyethylene glycols (PEGs) of various chain length were used tocrosslink lysozyme onto an insoluble support such as oxirane. A very high degree of modification and noinactivation of lysozyme were obtained with PEG 20000, but enzymatic activity increased up to 20 times at pH 3.0, at which point the activity of the native enzyme was lower when using Leuconos tok oenus as a macromolecular substrate.

Index Entries

Lysozyme enzymeimmobilization Eupergit C polyethylene glycol covalent attachment 

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Copyright information

© Humana Press Inc 1999

Authors and Affiliations

  • Marina Zacchigna
    • 1
  • Gabriella Di Luca
    • 1
  • Lucia Lassiani
    • 1
  • Antonio Varnavas
    • 1
  • Anna Pitotti
    • 2
  • Enrico Boccù
    • 1
    Email author
  1. 1.Department of Pharmaceutical ScienceUniversity of TriesteTriesteItaly
  2. 2.Department of Food ScienceUniversity of UdineUdineItaly

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