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Applied Biochemistry and Biotechnology

, Volume 76, Issue 3, pp 161–170 | Cite as

Production of laccase by immobilized cells of Agaricus sp.

Induction effect of xylan and lignin derivatives
  • V. M. Kaluskar
  • B. P. Kapadnis
  • C. Jaspers
  • M. J. PennickxEmail author
Article

Abstract

Laccase was produced in the supernatant of culture of a local isolate of Agaricus sp. obtained from decaying Ficus religiosa wood. The enzyme was produced at a constitutive level when growing the fungus in a nitrogenlimited medium supplemented with either glycerol, glucose, fructose, mannitol, arabinose, maltose, sacch arose, cellulose, or cellobiose. Atwo-to sixfold increase in enzyme specific activity was observed when growing the strain in the presence of straw, xylan, xylose, lignosulfonate, veratryl alcohol, and ferulic and veratric acid. Experimentsare consistent with the existence of an induction control on laccase and the absence of a form of carbon catabolite repression mediated by noninducing carbon sources. Immobilization of the Agaricus sp. on several supports, including polyurethane foam, textilestrips, and straw, resulted in an increase of enzyme production as compared to cultivation in liquid medium.

Index Entries

Agaricus white-rot fungi laccase polyphenol oxidase cell immobilization paper industry application 

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Copyright information

© Humana Press Inc 1999

Authors and Affiliations

  • V. M. Kaluskar
    • 1
  • B. P. Kapadnis
    • 2
  • C. Jaspers
    • 3
  • M. J. Pennickx
    • 3
    Email author
  1. 1.Department of Environmental Sciences, School of Environmental SciencesUniversity of PoonePooneIndia
  2. 2.Department of MicrobiologyUniversity of PoonePooneIndia
  3. 3.Microbial Physiology and Ecology, Section Interfacultaire d'AgronomieUniversité Libre de BruxellesBrusselsBelgium

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