Applied Biochemistry and Biotechnology

, Volume 76, Issue 3, pp 161–170 | Cite as

Production of laccase by immobilized cells of Agaricus sp.

Induction effect of xylan and lignin derivatives
  • V. M. Kaluskar
  • B. P. Kapadnis
  • C. Jaspers
  • M. J. PennickxEmail author


Laccase was produced in the supernatant of culture of a local isolate of Agaricus sp. obtained from decaying Ficus religiosa wood. The enzyme was produced at a constitutive level when growing the fungus in a nitrogenlimited medium supplemented with either glycerol, glucose, fructose, mannitol, arabinose, maltose, sacch arose, cellulose, or cellobiose. Atwo-to sixfold increase in enzyme specific activity was observed when growing the strain in the presence of straw, xylan, xylose, lignosulfonate, veratryl alcohol, and ferulic and veratric acid. Experimentsare consistent with the existence of an induction control on laccase and the absence of a form of carbon catabolite repression mediated by noninducing carbon sources. Immobilization of the Agaricus sp. on several supports, including polyurethane foam, textilestrips, and straw, resulted in an increase of enzyme production as compared to cultivation in liquid medium.

Index Entries

Agaricus white-rot fungi laccase polyphenol oxidase cell immobilization paper industry application 


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  1. 1.
    Eriksson, K. E. L., Blanchette, R. A., and Ander, P. (1990), Microbial and Enzymatic Degradation of Wood and Wood Components, Springer-Verlag, Berlin.Google Scholar
  2. 2.
    Gold, M. and Alic, M. (1993), Microbiol. Rev. 57, 605–622.Google Scholar
  3. 3.
    Webb, E. C. (1984), Enzyme Nomendat ure, Academic, Orlando, FL.Google Scholar
  4. 4.
    Thurston, C. F. (1994), Microbiodogy 140, 19–26.Google Scholar
  5. 5.
    Wood, D. A. and Goodenough, P. W. (1977), Arch. Microbiol. 114, 161–165.CrossRefGoogle Scholar
  6. 6.
    Wood, D. A. (1980), J. Gen. Microbiol. 117, 327–338.Google Scholar
  7. 7.
    Paice, M. J., Bourbonnais, R., and Reid, I. D. (1995), Tappi J. 77, 161–169.Google Scholar
  8. 8.
    Ainsworth, G. C. (1973), The Fungi: An Advanced Treatise vols. 4a and 4b, Ainsworth, G. C., Sparrow, K. F., and Sussman, A. S., eds., Academic, New York.Google Scholar
  9. 9.
    Kirk, T. K., Croan, S., Tien, M., Murtagh, K. E., and Farrell, R. L. (1986), Enzyme Microb. Technol. 8, 27–32.CrossRefGoogle Scholar
  10. 10.
    Chatel, M., Legon, T., Levert, J. M., Penninckx, M., and Vanthournhout, M. (1990), Physiology of Immobilized Cells, de Bont, J. A. M., Visser, J., Mattiasson, B., and Tramper, J., eds., Elsevier, Amsterdam, pp. 219–224.Google Scholar
  11. 11.
    Eggert, C., Temp, U., and Eriksson, K. E. (1996), Appl. Environ. Microbiol. 62, 1151–1158.Google Scholar
  12. 12.
    Srinivasan, C., D'Souza, T.M., Boominathan, K., and Reddy, C. A. (1995), Appl. Environ. Microbiol. 61, 4274–4277.Google Scholar
  13. 13.
    Daniels, L., Hanson, R. S., and Phillips, J. A. (1994), Methods for General and Molecular Bacteriology, Gerhardt, P., Murray, R. G. E., Wood, W. A., and Krieg, N., eds., American Society of Microbiology, Washington, DC, pp. 512–554.Google Scholar
  14. 14.
    Miller, G. L. (1959), Anal. Chem. 31, 426–428.CrossRefGoogle Scholar
  15. 15.
    Schmidt, J., John, M., and Wandrey, C. (1981), J. Chromatogr. 213, 151–155.CrossRefGoogle Scholar
  16. 16.
    Smith, J. F. (1993), Exploit at ion of Microorganisms, Gareth Jones, D., ed., Chapman & Hall, London, pp. 249–265.Google Scholar
  17. 17.
    Linko, S. (1988), Enzyme Microb. Technol. 10, 410–417.CrossRefGoogle Scholar
  18. 18.
    Munoz, C., Guillen, F., Martinez, A. T., and Martinez, M. J. (1997), Appl. Environ. Microbiol. 63, 2166–2174.Google Scholar
  19. 19.
    Yaver, D. S., Xu, F., Golightly, E. J., Brown, K. M., Brown, S. H., Rey, M. W., Schneider, P., Halkier, T., Mondorf, K., and Dalboge H. (1996), Appl. Environ. Microbiol. 62, 834–841.Google Scholar
  20. 20.
    Perry, C. R., Smith, M., Britnell, C. H., Wood, D. A., and Thurston C. (1993), J. Gen. Microbiol. 139, 1209–1218.Google Scholar
  21. 21.
    Bes, B., Pettersson, B., Lenholm, H., Iversen, T., and Eriksson, K. E. (1987), Biotechnol. Appl. Biochem. 9, 310–318.Google Scholar

Copyright information

© Humana Press Inc 1999

Authors and Affiliations

  • V. M. Kaluskar
    • 1
  • B. P. Kapadnis
    • 2
  • C. Jaspers
    • 3
  • M. J. Pennickx
    • 3
    Email author
  1. 1.Department of Environmental Sciences, School of Environmental SciencesUniversity of PoonePooneIndia
  2. 2.Department of MicrobiologyUniversity of PoonePooneIndia
  3. 3.Microbial Physiology and Ecology, Section Interfacultaire d'AgronomieUniversité Libre de BruxellesBrusselsBelgium

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