Skip to main content
SpringerLink
Log in

Laccase of Coriolus zonatus

Isolation, purification, and some physicochemical properties

  • Published:
Applied Biochemistry and Biotechnology Aims and scope Submit manuscript

Abstract

Laccase is one of the lignolytic enzymes found in liquid cultures of the fungus Coriolus zonatus in defined medium. The enzyme was isolated from culture liquid and characterized. Laccase from C. zonatus is a single-chain protein with a molecular mass of 60 kDa. Carbohydrate moiety of enzyme consisted of mannose, galactose and N-acetyl-glucosamine in a ratio of 6:2:0,6 respectively, and comprised 10% of the entiremolecule lsoelectric point was detected at pH 4.6. Laccase was found to have a pH optimum of 4.9 and temperature optimum of 55°C. Substrate specificity studies were conducted with catechol, K-ferrocyanide, hydroquinone, and sinapinic acid as substrates. The highest efficiency of catalysis was observed with sinapic acid as the substrate. The kinetic constants k cat and K28 of this reaction were 624 s−1 and 7 μM, respectively.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Higuchi, T. (1993), J. Biotechnol. 30, 1–8.

    Article  CAS  Google Scholar 

  2. Kirk, T. K. and Farrel, R. L. (1987), Annu. Rev. Microbiol. 41, 465–505.

    Article  CAS  Google Scholar 

  3. Kawai, S., Umezawa, T., Shimada, M., and Higuchi, T. (1988), FEBS Lett., 23, 306–311.

    Google Scholar 

  4. Bourbonnais, K. and Paice, M. G. (1990), FEBS Lett. 267, 99–102.

    Article  CAS  Google Scholar 

  5. Kawai, S., Umezawa, T., and Higuchi, T. (1989), Wood Res. 76, 10–16

    CAS  Google Scholar 

  6. Bourbonnais, K. and Paice, M. G. (1992), Appl. Microbiol. Biotechnol. 36, 823–827.

    Article  CAS  Google Scholar 

  7. Bollag, J. M., Shuttleworth, K. L., and Anderson, O. H. (1988), Appl. Environ. Microbiol. 54, 3086–3091.

    CAS  Google Scholar 

  8. Nicole, M., Chamberland, H., Geiger, J. P., Lecours, N., Valero, J., Rio, B., and Ouellette, G. B. (1992). Appl. Environ. Microbiol. 58, 1727–1739.

    CAS  Google Scholar 

  9. Osterman, L. A. (1985). Chromatography of Proteins and Nuclear Acids, 1st ed, Nauka, Moscow.

    Google Scholar 

  10. Westermeier, R. (1993), Electrophoresis in Practice, VCH Verlagsgesellschaft, Weinheim, Germany, p. 277.

    Google Scholar 

  11. Khorlin, A. Ya., Shivan, S. D., Markin, V. A., Nasonov, V. V., and Mrzayanova, M. N. (1986) Biorganic Chem. 12, 1203–1213.

    CAS  Google Scholar 

  12. Malkin, R., Malmstrom, B. G., and Vanngard, T. (1969), Eur. J. Biochem. 7, 253–259.

    Article  CAS  Google Scholar 

  13. Varfolomevev, S. D., Naki, A., Yaropolov, A. I., and Berezin, I. V. (1985), Biokhimia 50, 1411–1420.

    Google Scholar 

  14. Lowry, O. H., Rosebrough, N. J., Farr, I. L., and Randall, R. J. (1951), J. Biol. Chem., 193, 265–275.

    CAS  Google Scholar 

  15. Yaropolov, A. I., Skorobogat'ko, O. V., Vartanov, S. S., and Varfolomeyev, S. D. (1994), Appl. Biochem. Biotechnol. 49, 257–275.

    CAS  Google Scholar 

  16. Salas, C., Lobos, S., Larrain, J., Salas, L., Cullen, D., and Vicuna, R. (1995), Biotechnol. Appl. Biochem. 21, 323–333.

    CAS  Google Scholar 

  17. Reinhammar, B. (1984), in Copper Proteins and Copper Enzymes, Vol. 3, Lontie, R., ed., CRC Press, Boca Raton, FL, pp. 1–35.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Laboratory of Kinetics of Biochemical Processes, A.N. Bach Institute of Biochemistry, Russian Academy of Sciences, Leninsky pr. 33, 117071, Moscow, Russia

    O. V. Koroljova (Skorobogat'ko), E. V. Stepanova, V. I. Biniukov & A. I. Jaropolov

  2. V. L. Komarova Institute of Botany, Russian Academy of Sciences, Prof. Popov St. 2, 197376, St. Petersuburg, Russia

    V. P. Gavrilova

  3. The Lomonosov Moscow University, 119809, Moscow, Rusia

    S. D. Varfolomeyev

  4. Institute for Biochemistry and Molecular Physiology, University of Potsdam, D-14943, Luckenwalde, Germany

    F. Scheller & A. Makower

  5. c/o Max-Delbruck-Centrum for Molecular Medicine, Robert-Rossle St. 10, 0-1115, Berlin, Germany

    A. Otto

Authors
  1. O. V. Koroljova (Skorobogat'ko)
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. E. V. Stepanova
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. V. P. Gavrilova
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. V. I. Biniukov
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. A. I. Jaropolov
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. S. D. Varfolomeyev
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. F. Scheller
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. A. Makower
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. A. Otto
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to O. V. Koroljova (Skorobogat'ko).

Rights and permissions

Reprints and permissions

About this article

Cite this article

Koroljova (Skorobogat'ko), O.V., Stepanova, E.V., Gavrilova, V.P. et al. Laccase of Coriolus zonatus . Appl Biochem Biotechnol 76, 115–127 (1999). https://doi.org/10.1385/ABAB:76:2:115

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1385/ABAB:76:2:115

Index Entries

Search

Navigation