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Applied Biochemistry and Biotechnology

, Volume 76, Issue 2, pp 115–127 | Cite as

Laccase of Coriolus zonatus

Isolation, purification, and some physicochemical properties
  • O. V. Koroljova (Skorobogat'ko)
  • E. V. Stepanova
  • V. P. Gavrilova
  • V. I. Biniukov
  • A. I. Jaropolov
  • S. D. Varfolomeyev
  • F. Scheller
  • A. Makower
  • A. Otto
Article

Abstract

Laccase is one of the lignolytic enzymes found in liquid cultures of the fungus Coriolus zonatus in defined medium. The enzyme was isolated from culture liquid and characterized. Laccase from C. zonatus is a single-chain protein with a molecular mass of 60 kDa. Carbohydrate moiety of enzyme consisted of mannose, galactose and N-acetyl-glucosamine in a ratio of 6:2:0,6 respectively, and comprised 10% of the entiremolecule lsoelectric point was detected at pH 4.6. Laccase was found to have a pH optimum of 4.9 and temperature optimum of 55°C. Substrate specificity studies were conducted with catechol, K-ferrocyanide, hydroquinone, and sinapinic acid as substrates. The highest efficiency of catalysis was observed with sinapic acid as the substrate. The kinetic constants k cat and K28 of this reaction were 624 s−1 and 7 μM, respectively.

Index Entries

Laccase enzyme purification Coriolus zonatus substrate specificity thermostability pH stability 

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Copyright information

© Humana Press Inc 1999

Authors and Affiliations

  • O. V. Koroljova (Skorobogat'ko)
    • 1
  • E. V. Stepanova
    • 1
  • V. P. Gavrilova
    • 2
  • V. I. Biniukov
    • 1
  • A. I. Jaropolov
    • 1
  • S. D. Varfolomeyev
    • 3
  • F. Scheller
    • 4
  • A. Makower
    • 4
  • A. Otto
    • 5
  1. 1.Laboratory of Kinetics of Biochemical Processes, A.N. Bach Institute of BiochemistryRussian Academy of SciencesMoscowRussia
  2. 2.V. L. Komarova Institute of BotanyRussian Academy of SciencesSt. PetersuburgRussia
  3. 3.The Lomonosov Moscow UniversityMoscowRusia
  4. 4.Institute for Biochemistry and Molecular PhysiologyUniversity of PotsdamLuckenwaldeGermany
  5. 5.c/o Max-Delbruck-Centrum for Molecular MedicineBerlinGermany

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