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Studies on alkaline protease produced by Bacillus sp. NG312

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Abstract

An alkalophilic hyperproducer of alkaline protease, Bacillus sp. NG312, was isolated, and the enzyme showed maximum activity at pH 11.0 and 60°C. The temperature optimum was increased by 10°C in presence of Ca2+. The crudeenzyme was found to have half-life of 11 d at 37°C and maximum stability at pH 9.0–10.0. It also exhibited very good stability in presence of detergent components and some locally available commericial detergent powders.

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  1. Debendra K. Sahoo

    Present address: Department of Chemical Engineering, Massachusetts Institute of Technology, Room No. 56-454, 02139, Cambridge, MA

Authors and Affiliations

  1. Institute of Microbial Technology, Sector 39-A, 160 036, Chandigarh, India

    Jasvir S., Navdeep Gill, Gina Devasahayam & Debendra K. Sahoo

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  1. Jasvir S.
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  2. Navdeep Gill
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  3. Gina Devasahayam
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  4. Debendra K. Sahoo
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Correspondence to Debendra K. Sahoo.

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Jasvir, S., Gill, N., Devasahayam, G. et al. Studies on alkaline protease produced by Bacillus sp. NG312. Appl Biochem Biotechnol 76, 57–63 (1999). https://doi.org/10.1385/ABAB:76:1:57

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  • DOI: https://doi.org/10.1385/ABAB:76:1:57

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