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Resveratrol-induced cell inhibition of growth and apoptosis in MCF7 human breast cancer cells are associated with modulation of phosphorylated akt and caspase-9

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Abstract

Resveratrol (trans-3,4N,-5-trihydroxystilbene), a phytoalexin present in grapes and red wine, is emerging as a natural compound with potential anticancer properties. Here we show that resveratrol affects the growth of human breast cancer cell lines MCF7, MDA-MB-231, SK-BR-3, and Bcap-37 in a dose-dependent manner and that MCF7 is the most sensitive among the four cell lines. MCF7 cells treated with resveratrol showed typical characteristics of apoptosis including the poly (ADP-ribose) polymerase cleavage, TdT-mediated dUTP nick end labeling-positive staining, and morphologic changes. Phosphorylation of the oncogene product Akt was significantly reduced followed by decreased phosphorylation and increased processing of pro-caspase-9 on resveratrol treatment. These results indicate that resveratrol seems to exert its growth-inhibitory/apoptotic effect on the breast cancer cell line MCF7 via the Akt-caspase-9 pathway.

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Author notes

  1. Libo Yao

    Present address: Xi'an Greenpharma Natural Medicine Lab, 710075, Xi'an, China

Authors and Affiliations

  1. The Department of Biochemistry and Molecular Biology, The Fourth Military Medical University, 710032, Xi'an, China

    Yan Li, Junye Liu, Xinping Liu, Kefei Xing, Fuyang Li & Libo Yao

  2. The State Key Laboratory of Cancer Biology, The Fourth Military Medical University, 710032, Xi'an, China

    Yan Li, Junye Liu, Xinping Liu, Kefei Xing & Libo Yao

  3. Xi'an Greenpharma Natural Medicine Lab, 710075, Xi'an, China

    Yun Wang

Authors
  1. Yan Li
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  2. Junye Liu
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  3. Xinping Liu
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  6. Fuyang Li
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Li, Y., Liu, J., Liu, X. et al. Resveratrol-induced cell inhibition of growth and apoptosis in MCF7 human breast cancer cells are associated with modulation of phosphorylated akt and caspase-9. Appl Biochem Biotechnol 135, 181–192 (2006). https://doi.org/10.1385/ABAB:135:3:181

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  • DOI: https://doi.org/10.1385/ABAB:135:3:181

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