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Culture pH affects exopolysaccharide production in submerged mycelial culture of Ganoderma lucidum

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Abstract

In submerged culture of Ganoderma lucidum, the pH optimum for cell growth has been shown to be lower than that for exopolysaccharides (EPS) formation. Therefore, in the present study, a two-stage pH-control strategy was employed to maximize the productions of mycelial biomass and EPS. When compared, a batch culture without pH control had a maximum concentration of EPS and endopolysaccharides, which was much lower than those with pH control. Maximum mycelial growth (12.5 g/L) and EPS production (4.7 g/L) were achieved by shifting the controlled pH from 3.0 to 6.0 after day 4. The contrast between the controlled-pH process and uncontrolled pH was marked. By using various two-stage culture processes, it was also observed that culture pH has a significant affect on the yield of product, mycelial morphology, chemical composition, and molecular weight of EPS. A detailed observation of mycelial morphology revealed that the productive morphological form for EPS production was a dispersed pellet (controlled pH shifting from 3.0 to 6.0) rather than a compact pellet with a dense core area (controlled pH 4.5) or a feather-like pellet (controlled pH shifting from 6.0 to 3.0). Three different polysaccharides were obtained from each pH conditions, and their molecular weights and chemical compositions were significantly different.

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Authors and Affiliations

  1. Department of Biotechnology, Daegu University, Kyungsan, 712-714, Kyungbuk, Korea

    Hyun Mi Kim & Jong Won Yun

  2. Department of Bioenvironment Science, Daegu Haany University, Kyungsan, 712-715, Kyungbuk, Korea

    Moon Ki Park

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  1. Hyun Mi Kim
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  2. Moon Ki Park
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  3. Jong Won Yun
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Correspondence to Jong Won Yun.

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Kim, H.M., Park, M.K. & Yun, J.W. Culture pH affects exopolysaccharide production in submerged mycelial culture of Ganoderma lucidum . Appl Biochem Biotechnol 134, 249–262 (2006). https://doi.org/10.1385/ABAB:134:3:249

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  • DOI: https://doi.org/10.1385/ABAB:134:3:249

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