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Applied Biochemistry and Biotechnology

, Volume 134, Issue 1, pp 39–50 | Cite as

Effect of lipase immobilization on resolution of (R, S)-2-octanol in nonaqueous media using modified ultrastable-Y molecular sieve as support

  • Dazhang Dai
  • Liming XiaEmail author
Original Research Articles

Abstract

The lipase from Penicillium expansum PED-03 (PEL) was immobilized onto modified ultrastable-Y (USY) molecular sieve and the resolution of (R, S)-2-octanol was carried out in a bioreactor in nonaqueous media by the immobilized lipase. It was found that the conversion rate, enantiomeric excess (ee) value, and enantioselectivity (E) value of the resolution catalyzed by PEL immobilized on modified USY molecular sieve were much higher than those of the reaction catalyzed by free PEL and PEL immobilized on other supports. Immobilized on modified USY molecular sieve, the PEL exhibited obvious activity within a wider pH range and at a much higher temperature and showed a markedly enhanced stability against thermal inactivation, by which the suitable pH of the buffer used for immobilization could be “memorized.” The conversion rate of the reaction catalyzed by PEL immobilized on modified USY molecular sieve reached 48.84%, with excellent enantio-selectivity (avarege E value of eight batches >460) in nonaqueous media at “memorial” pH 9.5, 50°C for 24 h, demonstrating a good application potential in the production of optically pure (R, S)-2-octanol.

Index Entries

Modified ultrastable-Y molecular sieve immobilized lipase resolution 2-octanol catalytic property 

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Copyright information

© Humana Press Inc 2006

Authors and Affiliations

  1. 1.Department of Chemical Engineering and BioengineeringZhejiang UniversityHangzhouThe People’s Republic of China

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