Abstract
A strain with high poly-γ-glutamic acid (γ-PGA) production was isolated from fermented bean curd, a traditional Chinese food. The strain was named Bacillus subtilis ZJU-7 according to 16s rDNA sequencing and its taxonomic characters. The culture conditions for γ-PGA production were evaluated. The most suitable carbon and nitrogen sources were sucrose and tryptone, respectively. Exogenous l-glutamic acid was necessary for γ-PGA production, and the production of γ-PGA increased on the addition of l-glutamic acid to the medium. In the medium containing 60 g/L of sucrose, 60 g/L of tryptone, 80 g/L of l-glutamic acid, and 10 g/L of NaCl, the yield of γ-PGA reached 54.4 g/L after cultivation at 37°C for 24h, which was the highest γ-PGA production compared with values reported in the literature. The average molecular mass of γ-PGA produced was about 1.24×106 Daltons. B. subtilis ZJU-7 is genetically stable and can synthesize levan instead of γ-PGA without the addition of l-glutamic acid to the medium.
Similar content being viewed by others
References
Kadowaki, M. and Noguchi, T. (2001), Biosci. Biotechnol. Biochem. 65, 516–521.
Choi, H. J. and Kunioka, M. (1995), Radiat. Phys. Chem. 46, 175–179.
Ashiuchi, M., Tani, K., Soda, K., and Misono, H. (1998), J. Biochem. 123, 1156–1163.
Richie, S. M. C., Bachas, L. G., and Olin, T. (1999), Langmuir 15, 6346–6357.
Ivanovics, G. and Bruckner, V. (1937), Z. Immunitatsforsch. 90, 304–318.
Fugii, H. (2001), Nippon Nogeikagaku Kaishi 37, 407–411.
Bovarnick, M. (1942), J. Biol. Chem. 145, 415–424.
Goto, A. and Kunioka, M. (1992), Biosci. Biotechnol. Biochem. 63(1), 105–110.
Birr, G. A., Cromwick, A. M., and Gross, R. A. (1994), Int. J. Biol. Macromol. 16 (5), 265–275.
Sneath, P. H. A., Krieg, N. R., and Holt, J. G. (1986), Berg’s Manual of Systematic Bacteriology [M], vol. 12, Willams & Wilkins, Baltimore.
Hansen, B. M. and Hendriksen, N. B. (2001), Appl. Environ. Microbiol. 67, 185–189.
Ashiuchi, M., Tani, K., Soda, K., and Misono, H. (1998), J. Biochem. 123, 1156–1163.
Dubois, M., Gilles, K. A., Hamilton, J. K., Rebers, P. A., and Smith, F. (1956), Anal. Chem. 28, 350–356.
Kunioka, M. (1994), Appl. Microbiol. Biotechnol. 40, 867–872.
Lee, Y. H. and Lin, T. I. (1996), J. Chromatogr. B 681, 87–97.
Ito, Y. Tanaka, T. Ohmachi, T., and Asada, Y. (1996), Biosci. Biotechnol. Biochem. 60, 1239–1242.
Shih, I. L. and Yu, Y. T. (2005), Biotechnol. Lett. 27, 103–106.
Nagai, T., Phan Tran, L. S., Inatsu, Y. and Itoh, Y. (2000), J. Bacteriol. 182 2387–2392.
Kubota, H. (1993), Biosci. Biotechnol. Biochem. 57(7), 1212–1213.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Shi, F., Xu, Z. & Cen, P. Efficient production of poly-γ-glutamic acid by bacillus subtilis ZJU-7. Appl Biochem Biotechnol 133, 271–281 (2006). https://doi.org/10.1385/ABAB:133:3:271
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1385/ABAB:133:3:271