Applied Biochemistry and Biotechnology

, Volume 133, Issue 3, pp 203–210 | Cite as

Production of ceramide with Saccharomyces cerevisiae

  • Kyu Hyuk Kwun
  • Jung-heon Lee
  • Kyung-ho Rho
  • Hyun-shik Yun


The possibility of producing the biologically active material of the skin, ceramide, was studied using yeasts. The yeast strain that produced the most ceramide, Saccharomyces cerevisiae (KCCM 50515), was selected, and the optimal conditions for ceramide production were determined using shakeflask culture and batch fermentation. By measuring the production rate of ceramide at various pH values and temperatures, the optimal conditions for ceramide production were found to be pH 6.0 and 30°C. When heat shock was applied to the cells for 1 h by increasing the culture temperature from 30 to 40°C after cell growth, the amount of ceramide produced was increased 5.9-fold. A cell growth and ceramide production model was developed with Monod kinetics and the Leudecking-Piret model. It showed that ceramide production was increased when the cells were in the stationary phase.

Index Entries

Ceramide heat shock optimal condition Saccharomyces cerevisiae 


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Copyright information

© Humana Press Inc 2006

Authors and Affiliations

  • Kyu Hyuk Kwun
    • 1
  • Jung-heon Lee
    • 1
  • Kyung-ho Rho
    • 2
  • Hyun-shik Yun
    • 2
  1. 1.Department of Chemical EngineeringChosun UniversityKwangjuKorea
  2. 2.Department of Chemical EngineeringInha UniversityInchonKorea

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