Abstract
Trichoderma reesei FTKO-39 grown at 35°C for 5 d on wheat bran supplemented with MgCl2 and lactose as the carbon source produced two isozymes of β-galactosidase: BGT I and BGT II. These isozymes were partially purified on a DEAE-Trisacryl column. Both BGT I and BGT II fractions exhibited optimum activity at 65°C, but the pH optima were 4.0 and 6.5, respectively. The isozymes also showed similar thermal stability. However, BGT I was more stable than BGT II in a pH range of 3.0–10.0. At least two different β-galactosidases are produced by T. reesei, as revealed by the two bands seen on a 6% polyacrylamide gel stained for activity.
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Adalberto, P.R., Massabni, A.C., Goulart, A.J. et al. Production of β-galactosidase by Trichoderma reesei FTKO-39 in wheat bran. Appl Biochem Biotechnol 133, 163–170 (2006). https://doi.org/10.1385/ABAB:133:2:163
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DOI: https://doi.org/10.1385/ABAB:133:2:163