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Applied Biochemistry and Biotechnology

, Volume 132, Issue 1–3, pp 951–958 | Cite as

Inulinase production by Kluyveromyces marxianus NRRL Y-7571 using solid state fermentation

  • João Paulo Bender
  • Marcio Antônio Mazutti
  • Débora de Oliveira
  • Marco Di Luccio
  • Helen Treichel
Session 6 Bioprocess Research and Development

Abstract

Inulinase is an enzyme relevant to fructose production by enzymatic hydrolysis of inulin. This enzyme is also applied in the production of fructo-oligosaccharides that may be used as a new food functional ingredient. Commercial inulinase is currently obtained using inulin as substrate, which is a relatively expensive raw material. In Brazil, the production of this enzyme using residues of sugarcane and corn industry (sugarcane bagasse, molasses, and corn steep liquor) is economically attractive, owing to the high amount and low cost of such residues. In this context, the aim of this work was the assessment of inulinase production by solid state fermentation using by Kluyveromyces marxianus NRRL Y-7571. The solid medium consisted of sugar cane bagasse supplemented with molasses and corn steep liquor. The production of inulinase was carried out using experimental design technique. The effect of temperature, moisture, and supplements content were investigated. The enzymatic activity reached a maximum of 445 units of inulinase per gram of dry substrate.

Index Entries

Inulinase solid state Kluyveromyces marxianus 

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Copyright information

© Humana Press Inc 2006

Authors and Affiliations

  • João Paulo Bender
    • 1
  • Marcio Antônio Mazutti
    • 1
  • Débora de Oliveira
    • 1
  • Marco Di Luccio
    • 1
  • Helen Treichel
    • 1
  1. 1.Departmento de Engenharia de AlimentosUniversidade Regional Integrada do Alto Uruguai e das Missões-URI Campus de ErechimErechim

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