Applied Biochemistry and Biotechnology

, Volume 129, Issue 1–3, pp 278–288 | Cite as

Effect of inhibitors released during steam-explosion pretreatment of barley straw on enzymatic hydrolysis

  • Ma Prado García-Aparicio
  • Ignacio Ballesteros
  • Alberto González
  • José Miguel Oliva
  • Mercedes Ballesteros
  • Ma José Negro
Session 1B: Enzyme Catalysis and Engineering


The influence of the liquid fraction (prehydrolysate) generated during steam-explosion pretreatment (210°C, 15 min) of barley straw on the enzymatic hydrolysis was determined. Prehydrolysate was analyzed for degradation compounds and sugars' content and used as a medium for enzymatic hydrolysis tests after pH adjusting to 4.8. Our results show that the presence of the compounds contained in the prehydrolysate strongly affects the hydrolysis step (a 25% decrease in cellulose conversion compared with control). Sugars are shown to be more potent inhibitors of enzymatic hydrolysis than degradation products.

Index Entries

Enzymatic hydrolysis barley straw inhibition steam explosion 


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Copyright information

© Humana Press Inc. 2006

Authors and Affiliations

  • Ma Prado García-Aparicio
    • 1
  • Ignacio Ballesteros
    • 1
  • Alberto González
    • 1
  • José Miguel Oliva
    • 1
  • Mercedes Ballesteros
    • 1
  • Ma José Negro
    • 1
  1. 1.Renewable Energies Department—CIEMATMadridSpain

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