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Effect of pH and phosphate on trapping capacity of various heavy metal ions with ferritin reactor in flowing seawater

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Abstract

We describe a protein reactor consisting of native liver ferritin of Dasyatis akajei (DALF) and a dialysis bag. Our goal was to study a ferritin reactor for its capacity to trap various heavy metal ions (M2+) in flowing seawater. The reactor is sensitive and inexpensive and can be operated by nonprofessional technicians. A positive relationship between the number of trapped M2+ with the DALF reactor and its concentration in the flowing seawater was observed. Both the pH in the medium and the phosphate content within the ferritin cavity strongly affected trapping capacity. It was found that the ferritin released its phosphate compound directly with a shift in pH without the need for releasing reagent, which differs from the phosphate release characteristics of horse spleen ferritin, as previously described. This behavior evidently makes the trapping capacity with the ferritin reactor weaken, indicating that this trapping capacity is tightly connected to its phosphate compound. Our study shows that a self-regulation ability of the ferritin shell rather than its phosphate compound plays an important role in controlling the rate and capacity of trapping M2+. The ferritin reactor was constructed to monitor the contamination level of M2+ in flowing seawater. Our preliminary data along with fieldwork indicate that the DALF reactor is an analytical means for effectively monitoring the contamination level of M2+ in flowing seawater.

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Authors and Affiliations

  1. Department of Biochemistry and Biotechnology, School of Life Sciences, State Key Laboratory of Marine Environmental Science, Xiamen University, 361005, Xiamen, China

    Bo Kong, He-Qing Huang & Ping Chen

  2. The Key Laboratory for Chemical Biology of Fujian Province, Xiamen University, 361005, Xiamen, China

    He-Qing Huang & Qing-Mei Lin

  3. Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Hong Kong

    Zong-Wei Cai

Authors
  1. Bo Kong
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  2. He-Qing Huang
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  3. Qing-Mei Lin
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  4. Zong-Wei Cai
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  5. Ping Chen
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Correspondence to He-Qing Huang.

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Kong, B., Huang, HQ., Lin, QM. et al. Effect of pH and phosphate on trapping capacity of various heavy metal ions with ferritin reactor in flowing seawater. Appl Biochem Biotechnol 126, 133–148 (2005). https://doi.org/10.1385/ABAB:126:2:133

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  • DOI: https://doi.org/10.1385/ABAB:126:2:133

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