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Purification and characterization of two low molecular weight endoglucanases produced by Penicillium occitanis mutant pol 6

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Abstract

Two endoglucanases (EGs), EG A and EG B, were purified to homogeneity from Penicillium occitanis mutant Pol6 culture medium. The molecular weights of EGA and EGB were 31,000 and 28,000 kDa, respectively. The pI was about 3 for EG A and 7.5 for EG B. Optimal activity was obtained at pH 3.5 for both endoglucanases. Optimal temperature for enzyme activity was 60°C for EG A and 50°C for EG B. EG A was thermostable at 60°C and remained active after 1 h at 70°C. EGs hydrolyzed carboxymethylcellulose, phosphoric acid swollen cellulose, and ß-glucan efficiently, whereas microcrystalline cellulose (Avicel) and laminarin were poorly hydrolyzed. Only EG B showed xylanase activity. Furthermore, these EGs were insensitive to the action of glucose and cellobiose but were inhibited by the divalent cations Hg2+, Co2+, and Mn2+.

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Authors and Affiliations

  1. National School of Engineers of Sfax, BP W 3038, Stax Cedex, Tunisia

    Semia Ellouz Chaabouni

  2. Biotechnology Center of Sfax, BP W 3038, Sfax Cedex, Tunisia

    Tahar Mechichi

  3. National Institute of Scientific and Technical Research, PB 95, 2050, Hammam-Lif, Tunisia

    Ferid Limam

  4. National Institute of Applied Sciences and Technology, BP 676, 11080, Tunis Cedex, Tunisia

    Nejib Marzouki

Authors
  1. Semia Ellouz Chaabouni
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  2. Tahar Mechichi
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  3. Ferid Limam
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  4. Nejib Marzouki
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Correspondence to Semia Ellouz Chaabouni.

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Chaabouni, S.E., Mechichi, T., Limam, F. et al. Purification and characterization of two low molecular weight endoglucanases produced by Penicillium occitanis mutant pol 6. Appl Biochem Biotechnol 125, 99–112 (2005). https://doi.org/10.1385/ABAB:125:2:099

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  • DOI: https://doi.org/10.1385/ABAB:125:2:099

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